1f5s

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1f5s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f5s, resolution 1.8&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
Current revision (13:23, 13 March 2024) (edit) (undo)
 
(18 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1f5s.gif|left|200px]]<br /><applet load="1f5s" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1f5s, resolution 1.8&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF PHOSPHOSERINE PHOSPHATASE FROM METHANOCOCCUS JANNASCHII'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF PHOSPHOSERINE PHOSPHATASE FROM METHANOCOCCUS JANNASCHII==
-
BACKGROUND: D-Serine is a co-agonist of the N-methyl-D-aspartate subtype, of glutamate receptors, a major neurotransmitter receptor family in, mammalian nervous systems. D-Serine is converted from L-serine, 90% of, which is the product of the enzyme phosphoserine phosphatase (PSP). PSP, from M. jannaschii (MJ) shares significant sequence homology with human, PSP. PSPs and P-type ATPases are members of the haloacid dehalogenase, (HAD)-like hydrolase family, and all members share three conserved, sequence motifs. PSP and P-type ATPases utilize a common mechanism that, involves Mg(2+)-dependent phosphorylation and autodephosphorylation at an, aspartyl side chain in the active site. The strong resemblance in sequence, and mechanism implies structural similarity among these enzymes. RESULTS:, The PSP crystal structure resembles the NAD(P) binding Rossmann fold with, a large insertion of a four-helix-bundle domain and a beta hairpin. Three, known conserved sequence motifs are arranged next to each other in space, and outline the active site. A phosphate and a magnesium ion are bound to, the active site. The active site is within a closed environment between, the core alpha/beta domain and the four-helix-bundle domain. CONCLUSIONS:, The crystal structure of MJ PSP was determined at 1.8 A resolution., Critical residues were assigned based on the active site structure and, ligand binding geometry. The PSP structure is in a closed conformation, that may resemble the phosphoserine bound state or the state after, autodephosphorylation. Compared to a P-type ATPase (Ca(2+)-ATPase), structure, which is in an open state, this PSP structure appears also to, be a good model for the closed conformation of P-type ATPase.
+
<StructureSection load='1f5s' size='340' side='right'caption='[[1f5s]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1f5s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F5S FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f5s OCA], [https://pdbe.org/1f5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f5s RCSB], [https://www.ebi.ac.uk/pdbsum/1f5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f5s ProSAT], [https://www.topsan.org/Proteins/BSGC/1f5s TOPSAN]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SERB_METJA SERB_METJA]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f5/1f5s_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f5s ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1F5S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with PO4 and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoserine_phosphatase Phosphoserine phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.3 3.1.3.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F5S OCA].
+
*[[Phosphoserine phosphatase|Phosphoserine phosphatase]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure of phosphoserine phosphatase from Methanococcus jannaschii, a hyperthermophile, at 1.8 A resolution., Wang W, Kim R, Jancarik J, Yokota H, Kim SH, Structure. 2001 Jan 10;9(1):65-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11342136 11342136]
+
[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
-
[[Category: Phosphoserine phosphatase]]
+
[[Category: Jancarik J]]
-
[[Category: Single protein]]
+
[[Category: Kim R]]
-
[[Category: BSGC, Berkeley.Structural.Genomics.Center.]]
+
[[Category: Kim SH]]
-
[[Category: Jancarik, J.]]
+
[[Category: Wang W]]
-
[[Category: Kim, R.]]
+
[[Category: Yokota H]]
-
[[Category: Kim, S.H.]]
+
-
[[Category: Wang, W.]]
+
-
[[Category: Yokota, H.]]
+
-
[[Category: MG]]
+
-
[[Category: PO4]]
+
-
[[Category: berkeley structural genomics center]]
+
-
[[Category: beta-hair pin]]
+
-
[[Category: bsgc structure funded by nih]]
+
-
[[Category: four helix bundle]]
+
-
[[Category: had family hydrolase]]
+
-
[[Category: nad(p)-binding rossmann fold]]
+
-
[[Category: protein structure initiative]]
+
-
[[Category: psi]]
+
-
[[Category: structural genomics]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:38:23 2007''
+

Current revision

CRYSTAL STRUCTURE OF PHOSPHOSERINE PHOSPHATASE FROM METHANOCOCCUS JANNASCHII

PDB ID 1f5s

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1f5s"
Personal tools