1f6k

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(New page: 200px<br /><applet load="1f6k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f6k, resolution 1.60&Aring;" /> '''CRYSTAL STRUCTURE AN...)
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[[Image:1f6k.jpg|left|200px]]<br /><applet load="1f6k" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f6k, resolution 1.60&Aring;" />
 
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'''CRYSTAL STRUCTURE ANALYSIS OF N-ACETYLNEURAMINATE LYASE FROM HAEMOPHILUS INFLUENZAE: CRYSTAL FORM II'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE ANALYSIS OF N-ACETYLNEURAMINATE LYASE FROM HAEMOPHILUS INFLUENZAE: CRYSTAL FORM II==
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The N-acetylneuraminate lyase (NAL) sub-family of (beta/alpha)(8) enzymes, share a common catalytic step but catalyse reactions in different, biological pathways. Known examples include NAL, dihydrodipicolinate, synthetase (DHDPS), d-5-keto-4-deoxyglucarate dehydratase, 2-keto-3-deoxygluconate aldolase, trans-o-hydroxybenzylidenepyruvate, hydrolase-aldolase and trans-2'-carboxybenzalpyruvate hydratase-aldolase., Little is known about the way in which the three-dimensional structure of, the respective active sites are modulated across the sub-family to achieve, cognate substrate recognition. We present here the structure of, Haemophilus influenzae NAL determined by X-ray crystallography to a, maximum resolution of 1.60 A, in native form and in complex with three, substrate analogues (sialic acid alditol, 4-deoxy-sialic acid and, 4-oxo-sialic acid). These structures reveal for the first time the mode of, binding of the complete substrate in the NAL active site. On the basis of, the above structures, that of substrate-complexed DHDPS and sequence, comparison across the sub-family we are able to propose a unified model, for active site modulation. The model is one of economy, allowing wherever, appropriate the retention or relocation of residues associated with, binding common substrate substituent groups. Our structures also suggest a, role for the strictly conserved tyrosine residue found in all active sites, of the sub-family, namely that it mediates proton abstraction by the, alpha-keto acid carboxylate in a substrate-assisted catalytic reaction, pathway.
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<StructureSection load='1f6k' size='340' side='right'caption='[[1f6k]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1f6k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F6K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f6k OCA], [https://pdbe.org/1f6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f6k RCSB], [https://www.ebi.ac.uk/pdbsum/1f6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f6k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NANA_HAEIN NANA_HAEIN] Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/1f6k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f6k ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1F6K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/N-acetylneuraminate_lyase N-acetylneuraminate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.3 4.1.3.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F6K OCA].
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*[[N-acetylneuraminate lyase 3D structures|N-acetylneuraminate lyase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Active site modulation in the N-acetylneuraminate lyase sub-family as revealed by the structure of the inhibitor-complexed Haemophilus influenzae enzyme., Barbosa JA, Smith BJ, DeGori R, Ooi HC, Marcuccio SM, Campi EM, Jackson WR, Brossmer R, Sommer M, Lawrence MC, J Mol Biol. 2000 Oct 27;303(3):405-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11031117 11031117]
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[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: N-acetylneuraminate lyase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Barbosa JARG]]
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[[Category: Barbosa, J.A.R.G.]]
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[[Category: DeGori R]]
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[[Category: DeGori, R.]]
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[[Category: Lawrence MC]]
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[[Category: Lawrence, M.C.]]
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[[Category: Smith BJ]]
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[[Category: Smith, B.J.]]
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[[Category: GOL]]
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[[Category: SO4]]
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[[Category: beta barrel]]
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[[Category: lyase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:39:27 2007''
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Current revision

CRYSTAL STRUCTURE ANALYSIS OF N-ACETYLNEURAMINATE LYASE FROM HAEMOPHILUS INFLUENZAE: CRYSTAL FORM II

PDB ID 1f6k

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