1f7d

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(New page: 200px<br /><applet load="1f7d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1f7d, resolution 1.40&Aring;" /> '''CRYSTAL STRUCTURES O...)
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[[Image:1f7d.gif|left|200px]]<br /><applet load="1f7d" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1f7d, resolution 1.40&Aring;" />
 
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'''CRYSTAL STRUCTURES OF FELINE IMMUNODEFICIENCY VIRUS DUTP PYROPHOSPHATASE AND ITS NUCLEOTIDE COMPLEXES IN THREE CRYSTAL FORMS'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURES OF FELINE IMMUNODEFICIENCY VIRUS DUTP PYROPHOSPHATASE AND ITS NUCLEOTIDE COMPLEXES IN THREE CRYSTAL FORMS==
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dUTP pyrophosphatase (dUTPase) cleaves the alpha-beta phosphodiester of, dUTP to form pyrophosphate and dUMP, preventing incorporation of uracil, into DNA and providing the substrate for thymine synthesis. Seven crystal, structures of feline immunodeficiency virus (FIV) dUTPase in three crystal, forms have been determined, including complexes with substrate (dUTP), product (dUMP) or inhibitor (dUDP) bound. The native enzyme has been, refined at 1.40 A resolution in a hexagonal crystal form and at 2.3 A, resolution in an orthorhombic crystal form. In the dUDP complex in a cubic, crystal form refined at 2.5 A resolution, the C-terminal conserved P-loop, motif is fully ordered. The analysis defines the roles of five sequence, motifs in interaction with uracil, deoxyribose and the alpha-, beta- and, gamma-phosphates. The enzyme utilizes adaptive recognition to bind the, alpha- and beta-phosphates. In particular, the alpha-beta phosphodiester, adopts an unfavorable eclipsed conformation in the presence of the P-loop., This conformation may be relevant to the mechanism of alpha-beta, phosphodiester bond cleavage.
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<StructureSection load='1f7d' size='340' side='right'caption='[[1f7d]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1f7d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Feline_immunodeficiency_virus Feline immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F7D FirstGlance]. <br>
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1F7D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Feline_immunodeficiency_virus Feline immunodeficiency virus] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F7D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f7d OCA], [https://pdbe.org/1f7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f7d RCSB], [https://www.ebi.ac.uk/pdbsum/1f7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f7d ProSAT]</span></td></tr>
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Structures of feline immunodeficiency virus dUTP pyrophosphatase and its nucleotide complexes in three crystal forms., Prasad GS, Stura EA, Elder JH, Stout CD, Acta Crystallogr D Biol Crystallogr. 2000 Sep;56(Pt 9):1100-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10957629 10957629]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/POL_FIVPE POL_FIVPE] During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/1f7d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f7d ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Feline immunodeficiency virus]]
[[Category: Feline immunodeficiency virus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: dUTP diphosphatase]]
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[[Category: Elder JH]]
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[[Category: Elder, J.H.]]
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[[Category: Prasad GS]]
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[[Category: Prasad, G.S.]]
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[[Category: Stout CD]]
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[[Category: Stout, C.D.]]
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[[Category: Stura EA]]
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[[Category: Stura, E.A.]]
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[[Category: MG]]
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[[Category: eight stranded beta-barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:40:36 2007''
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Current revision

CRYSTAL STRUCTURES OF FELINE IMMUNODEFICIENCY VIRUS DUTP PYROPHOSPHATASE AND ITS NUCLEOTIDE COMPLEXES IN THREE CRYSTAL FORMS

PDB ID 1f7d

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