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1f8i

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CRYSTAL STRUCTURE OF ISOCITRATE LYASE:NITROPROPIONATE:GLYOXYLATE COMPLEX FROM MYCOBACTERIUM TUBERCULOSIS

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Retrieved from "http://52.214.119.220/wiki/index.php/1f8i"

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-[[Image:1f8i.gif|left|200px]]<br /><applet load="1f8i" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1f8i, resolution 2.25&Aring;" />
-'''CRYSTAL STRUCTURE OF ISOCITRATE LYASE:NITROPROPIONATE:GLYOXYLATE COMPLEX FROM MYCOBACTERIUM TUBERCULOSIS'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF ISOCITRATE LYASE:NITROPROPIONATE:GLYOXYLATE COMPLEX FROM MYCOBACTERIUM TUBERCULOSIS==
-Isocitrate lyase (ICL) plays a pivotal role in the persistence of, Mycobacterium tuberculosis in mice by sustaining intracellular infection, in inflammatory macrophages. The enzyme allows net carbon gain by, diverting acetyl-CoA from beta-oxidation of fatty acids into the, glyoxylate shunt pathway. Given its potential as a drug target against, persistent infections, we solved its structure without ligand and in, complex with two inhibitors. Covalent modification of an active site, residue, Cys 191, by the inhibitor 3-bromopyruvate traps the enzyme in a, catalytic conformation with the active site completely inaccessible to, solvent. The structure of a C191S mutant of the enzyme with the inhibitor, 3-nitropropionate provides further insight into the reaction mechanism.
+<StructureSection load='1f8i' size='340' side='right'caption='[[1f8i]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1f8i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F8I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F8I FirstGlance]. <br>
-F8I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with MG, GLV and SIN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Isocitrate_lyase Isocitrate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.3.1 4.1.3.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1F8I OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLV:GLYOXYLIC+ACID'>GLV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f8i OCA], [https://pdbe.org/1f8i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f8i RCSB], [https://www.ebi.ac.uk/pdbsum/1f8i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f8i ProSAT]</span></td></tr>
-Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis., Sharma V, Sharma S, Hoener zu Bentrup K, McKinney JD, Russell DG, Jacobs WR Jr, Sacchettini JC, Nat Struct Biol. 2000 Aug;7(8):663-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10932251 10932251]
+</table>
-[[Category: Isocitrate lyase]]
+== Function ==
-[[Category: Mycobacterium tuberculosis]]
+[https://www.uniprot.org/uniprot/ACEA_MYCTU ACEA_MYCTU] Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase-positive serine pathway (By similarity).
-[[Category: Single protein]]
+== Evolutionary Conservation ==
-[[Category: Bentrup, K.Hoener.zu.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Jr., W.R.Jacobs.]]
+Check<jmol>
-[[Category: McKinney, J.D.]]
+  <jmolCheckbox>
-[[Category: Russell, D.G.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f8/1f8i_consurf.spt"</scriptWhenChecked>
-[[Category: Sacchettini, J.C.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Sharma, S.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: Sharma, V.]]
+  </jmolCheckbox>
-[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f8i ConSurf].
-[[Category: GLV]]
+<div style="clear:both"></div>
-[[Category: MG]]
+__TOC__
-[[Category: SIN]]
+</StructureSection>
-[[Category: alpha-beta barrel]]
+[[Category: Large Structures]]
-[[Category: closed conformation]]
+[[Category: Mycobacterium tuberculosis H37Rv]]
-[[Category: protein structure initiative]]
+[[Category: Hoener zu Bentrup K]]
-[[Category: psi]]
+[[Category: Jacobs Jr WR]]
-[[Category: structural genomics]]
+[[Category: McKinney JD]]
-[[Category: swapped helices]]
+[[Category: Russell DG]]
-[[Category: tb structural genomics consortium]]
+[[Category: Sacchettini JC]]
-[[Category: tbsgc]]
+[[Category: Sharma S]]
+[[Category: Sharma V]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:42:26 2007''

1f8i

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CRYSTAL STRUCTURE OF ISOCITRATE LYASE:NITROPROPIONATE:GLYOXYLATE COMPLEX FROM MYCOBACTERIUM TUBERCULOSIS

Structural highlights

Function

Evolutionary Conservation

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