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1fba

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(New page: 200px<br /><applet load="1fba" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fba, resolution 1.9&Aring;" /> '''THE CRYSTAL STRUCTURE...)
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[[Image:1fba.gif|left|200px]]<br /><applet load="1fba" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fba, resolution 1.9&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE FROM DROSOPHILA MELANOGASTER AT 2.5 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE FROM DROSOPHILA MELANOGASTER AT 2.5 ANGSTROMS RESOLUTION==
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The structure of fructose-1,6-bisphosphate aldolase from Drosophila, melanogaster has been determined by X-ray diffraction at 2.5 A resolution., The insect enzyme crystallizes in space group P2(1)2(1)2(1) with lattice, replacement with rabbit muscle aldolase as a search model has been, employed to solve the structure. To improve the initial phases real space, averaging, including phase extension from 4.0 to 2.5 A, has been applied., Refinement of the atomic positions by molecular dynamics resulted in a, crystallographic R-factor of 0.214. The tertiary structure resembles in, most parts that of the vertebrate aldolase from rabbit muscle. Significant, differences were found in surface loops and the N- and C-terminal regions, of the protein. Here we present the first aldolase structure where the, functionally important C-terminal arm is described completely.
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<StructureSection load='1fba' size='340' side='right'caption='[[1fba]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fba]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FBA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FBA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fba OCA], [https://pdbe.org/1fba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fba RCSB], [https://www.ebi.ac.uk/pdbsum/1fba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fba ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALF_DROME ALF_DROME] May take part in developmental stage-specific or tissue -specific sugar-phosphate metabolisms. Protein acts on two substrates fructose 1,6-bisphosphate and fructose 1-phosphate (like other class I aldolases).<ref>PMID:8537310</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/1fba_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fba ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FBA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ACE as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FBA OCA].
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*[[Aldolase 3D structures|Aldolase 3D structures]]
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== References ==
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==Reference==
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<references/>
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The crystal structure of fructose-1,6-bisphosphate aldolase from Drosophila melanogaster at 2.5 A resolution., Hester G, Brenner-Holzach O, Rossi FA, Struck-Donatz M, Winterhalter KH, Smit JD, Piontek K, FEBS Lett. 1991 Nov 4;292(1-2):237-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1959612 1959612]
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Fructose-bisphosphate aldolase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Brenner-Holzach O]]
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[[Category: Brenner-Holzach, O.]]
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[[Category: Hester G]]
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[[Category: Hester, G.]]
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[[Category: Piontek K]]
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[[Category: Piontek, K.]]
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[[Category: ACE]]
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[[Category: lyase(aldehyde)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:46:59 2007''
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Current revision

THE CRYSTAL STRUCTURE OF FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE FROM DROSOPHILA MELANOGASTER AT 2.5 ANGSTROMS RESOLUTION

PDB ID 1fba

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