1fbx

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(New page: 200px<br /><applet load="1fbx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fbx, resolution 2.80&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1fbx.gif|left|200px]]<br /><applet load="1fbx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fbx, resolution 2.80&Aring;" />
 
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'''CRYSTAL STRUCTURE OF ZINC-CONTAINING E.COLI GTP CYCLOHYDROLASE I'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF ZINC-CONTAINING E.COLI GTP CYCLOHYDROLASE I==
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The crystal structure of recombinant human GTP cyclohydrolase I was solved, by Patterson search methods by using the coordinates of the Escherichia, coli enzyme as a model. The human as well as bacterial enzyme were shown, to contain an essential zinc ion coordinated to a His side chain and two, thiol groups in each active site of the homodecameric enzymes that had, escaped detection during earlier studies of the E. coli enzyme. The zinc, ion is proposed to generate a hydroxyl nucleophile for attack of imidazole, ring carbon atom eight of the substrate, GTP. It may also be involved in, the hydrolytic release of formate from the intermediate, 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate, and in the consecutive Amadori rearrangement of the ribosyl moiety.
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<StructureSection load='1fbx' size='340' side='right'caption='[[1fbx]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fbx]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FBX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fbx OCA], [https://pdbe.org/1fbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fbx RCSB], [https://www.ebi.ac.uk/pdbsum/1fbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fbx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCH1_ECOLI GCH1_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/1fbx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fbx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of recombinant human GTP cyclohydrolase I was solved by Patterson search methods by using the coordinates of the Escherichia coli enzyme as a model. The human as well as bacterial enzyme were shown to contain an essential zinc ion coordinated to a His side chain and two thiol groups in each active site of the homodecameric enzymes that had escaped detection during earlier studies of the E. coli enzyme. The zinc ion is proposed to generate a hydroxyl nucleophile for attack of imidazole ring carbon atom eight of the substrate, GTP. It may also be involved in the hydrolytic release of formate from the intermediate, 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate, and in the consecutive Amadori rearrangement of the ribosyl moiety.
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==About this Structure==
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Zinc plays a key role in human and bacterial GTP cyclohydrolase I.,Auerbach G, Herrmann A, Bracher A, Bader G, Gutlich M, Fischer M, Neukamm M, Garrido-Franco M, Richardson J, Nar H, Huber R, Bacher A Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13567-72. PMID:11087827<ref>PMID:11087827</ref>
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1FBX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/GTP_cyclohydrolase_I GTP cyclohydrolase I], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.16 3.5.4.16] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FBX OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Zinc plays a key role in human and bacterial GTP cyclohydrolase I., Auerbach G, Herrmann A, Bracher A, Bader G, Gutlich M, Fischer M, Neukamm M, Garrido-Franco M, Richardson J, Nar H, Huber R, Bacher A, Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13567-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11087827 11087827]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1fbx" style="background-color:#fffaf0;"></div>
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[[Category: GTP cyclohydrolase I]]
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[[Category: Single protein]]
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[[Category: Auerbach, G.]]
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[[Category: Bacher, A.]]
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[[Category: Bader, A.]]
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[[Category: Bracher, A.]]
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[[Category: Fischer, M.]]
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[[Category: Garrido-Franco, M.]]
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[[Category: Gutlich, M.]]
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[[Category: Herrmann, A.]]
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[[Category: Huber, R.]]
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[[Category: Nar, H.]]
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[[Category: Neukamm, M.]]
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[[Category: Richardson, J.]]
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[[Category: CL]]
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[[Category: ZN]]
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[[Category: allosteric enzyme]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:48:24 2007''
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==See Also==
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*[[Cyclohydrolase 3D structures|Cyclohydrolase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Auerbach G]]
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[[Category: Bacher A]]
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[[Category: Bader A]]
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[[Category: Bracher A]]
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[[Category: Fischer M]]
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[[Category: Garrido-Franco M]]
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[[Category: Gutlich M]]
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[[Category: Herrmann A]]
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[[Category: Huber R]]
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[[Category: Nar H]]
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[[Category: Neukamm M]]
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[[Category: Richardson J]]

Current revision

CRYSTAL STRUCTURE OF ZINC-CONTAINING E.COLI GTP CYCLOHYDROLASE I

PDB ID 1fbx

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