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1fcp

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(New page: 200px<br /><applet load="1fcp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fcp, resolution 2.70&Aring;" /> '''FERRIC HYDROXAMATE U...)
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[[Image:1fcp.gif|left|200px]]<br /><applet load="1fcp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fcp, resolution 2.70&Aring;" />
 
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'''FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI IN COMPLEX WITH BOUND FERRICHROME-IRON'''<br />
 
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==Overview==
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==FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI IN COMPLEX WITH BOUND FERRICHROME-IRON==
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FhuA, the receptor for ferrichrome-iron in Escherichia coli, is a member, of a family of integral outer membrane proteins, which, together with the, energy-transducing protein TonB, mediate the active transport of ferric, siderophores across the outer membrane of Gram-negative bacteria. The, three-dimensional structure of FhuA is presented here in two, conformations: with and without ferrichrome-iron at resolutions of 2.7 and, 2.5 angstroms, respectively. FhuA is a beta barrel composed of 22, antiparallel beta strands. In contrast to the typical trimeric arrangement, found in porins, FhuA is monomeric. Located within the beta barrel is a, structurally distinct domain, the "cork," which mainly consists of a, four-stranded beta sheet and four short alpha helices. A single, lipopolysaccharide molecule is noncovalently associated with the, membrane-embedded region of the protein. Upon binding of ferrichrome-iron, conformational changes are transduced to the periplasmic pocket of FhuA, signaling the ligand-loaded status of the receptor. Sequence homologies, and mutagenesis data are used to propose a structural mechanism for, TonB-dependent siderophore-mediated transport across the outer membrane.
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<StructureSection load='1fcp' size='340' side='right'caption='[[1fcp]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fcp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FCP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AAE:ACETOACETIC+ACID'>AAE</scene>, <scene name='pdbligand=EA2:AMINOETHANOLPYROPHOSPHATE'>EA2</scene>, <scene name='pdbligand=FCI:FERRICROCIN-IRON'>FCI</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GMH:L-GLYCERO-D-MANNO-HEPTOPYRANOSE'>GMH</scene>, <scene name='pdbligand=GP1:GLUCOSAMINE+1-PHOSPHATE'>GP1</scene>, <scene name='pdbligand=GP4:GLUCOSAMINE+4-PHOSPHATE'>GP4</scene>, <scene name='pdbligand=GPH:4-O-PHOSPHONO-D-GLYCERO-BETA-D-MANNO-HEPTOPYRANOSE'>GPH</scene>, <scene name='pdbligand=KDO:3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID'>KDO</scene>, <scene name='pdbligand=LIL:2-TRIDECANOYLOXY-PENTADECANOIC+ACID'>LIL</scene>, <scene name='pdbligand=LIM:3-OXO-PENTADECANOIC+ACID'>LIM</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fcp OCA], [https://pdbe.org/1fcp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fcp RCSB], [https://www.ebi.ac.uk/pdbsum/1fcp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fcp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FHUA_ECOLI FHUA_ECOLI] This receptor binds the ferrichrome-iron ligand. It interacts with the TonB protein, which is responsible for energy coupling of the ferrichrome-promoted iron transport system. Acts as a receptor for bacteriophage T5 as well as T1, phi80 and colicin M. Binding of T5 triggers the opening of a high conductance ion channel. Can also transport the antibiotic albomycin.<ref>PMID:8617231</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/1fcp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fcp ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FCP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4, NI, LIL, AAE, LIM, EA2 and FCI as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FCP OCA].
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*[[Ferric hydroxamate uptake receptor|Ferric hydroxamate uptake receptor]]
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== References ==
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==Reference==
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<references/>
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Siderophore-mediated iron transport: crystal structure of FhuA with bound lipopolysaccharide., Ferguson AD, Hofmann E, Coulton JW, Diederichs K, Welte W, Science. 1998 Dec 18;282(5397):2215-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9856937 9856937]
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__TOC__
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[[Category: Escherichia coli]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Escherichia coli K-12]]
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[[Category: Diederichs, K.]]
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[[Category: Large Structures]]
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[[Category: Ferguson, A.D.]]
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[[Category: Diederichs K]]
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[[Category: Hofmann, E.]]
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[[Category: Ferguson AD]]
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[[Category: Welte, W.]]
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[[Category: Hofmann E]]
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[[Category: AAE]]
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[[Category: Welte W]]
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[[Category: EA2]]
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[[Category: FCI]]
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[[Category: LIL]]
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[[Category: LIM]]
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[[Category: NI]]
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[[Category: PO4]]
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[[Category: active transport]]
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[[Category: ferrichrome-iron receptor]]
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[[Category: integral outer membrane protein ]]
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[[Category: iron transport protein]]
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[[Category: tonb-dependent receptor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:49:21 2007''
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Current revision

FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI IN COMPLEX WITH BOUND FERRICHROME-IRON

PDB ID 1fcp

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