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1fer

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(New page: 200px<br /><applet load="1fer" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fer, resolution 2.3&Aring;" /> '''STRUCTURE AT PH 6.5 O...)
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[[Image:1fer.jpg|left|200px]]<br /><applet load="1fer" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fer, resolution 2.3&Aring;" />
 
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'''STRUCTURE AT PH 6.5 OF FERREDOXIN I FROM AZOTOBACTER VINELANDII AT 2.3 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==STRUCTURE AT PH 6.5 OF FERREDOXIN I FROM AZOTOBACTER VINELANDII AT 2.3 ANGSTROMS RESOLUTION==
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Ferredoxin I from Azotobacter vinelandii (AvFdI) is an iron-sulfur protein, composed of 106 amino acids, seven Fe atoms and eight inorganic S* atoms., A crystallographic redetermination of its structure showed the originally, reported structure to be incorrect. We report here the crystal structure, of AvFdI at pH 6.5. Extensive refinement has led to a final R value of, 0.170 for all 6986 non-extinct reflections in the range 10-2.3 A using a, solvent model which includes 98 discrete solvent atoms with occupancies, between 0.3 and 1.0 and an average B value of 22.5 A(2). The first half of, the peptide chain closely resembles that of the 55-residue ferredoxin from, Peptococcus aerogenes (PaFd), while the remainder consists of three turns, of helix and a series of loops which form a cap over part of the molecular, core. Despite the similarities in structure and surroundings, the, corresponding 4Fe4S* clusters in PaFd and AvFdI have strikingly different, redox potentials; a possible explanation has been sought in the differing, hydration models for the two molecules.
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<StructureSection load='1fer' size='340' side='right'caption='[[1fer]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fer]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FER FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fer FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fer OCA], [https://pdbe.org/1fer PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fer RCSB], [https://www.ebi.ac.uk/pdbsum/1fer PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fer ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FER1_AZOVI FER1_AZOVI] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. This ferredoxin could play a role in regulating gene expression by interacting directly with DNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/1fer_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fer ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FER is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with F3S and SF4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FER OCA].
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*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure at pH 6.5 of ferredoxin I from Azotobacter vinelandii at 2.3 A resolution., Merritt EA, Stout GH, Turley S, Sieker LC, Jehsen LH, Orme-Johnson WH, Acta Crystallogr D Biol Crystallogr. 1993 Mar 1;49(Pt 2):272-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299532 15299532]
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[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Jensen, L.H.]]
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[[Category: Jensen LH]]
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[[Category: Merritt, E.A.]]
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[[Category: Merritt EA]]
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[[Category: Orme-Johnson, W.H.]]
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[[Category: Orme-Johnson WH]]
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[[Category: Sieker, L.C.]]
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[[Category: Sieker LC]]
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[[Category: Stout, G.H.]]
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[[Category: Stout GH]]
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[[Category: Turley, S.]]
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[[Category: Turley S]]
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[[Category: F3S]]
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[[Category: SF4]]
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[[Category: electron transfer(iron-sulfur protein)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:51:38 2007''
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Current revision

STRUCTURE AT PH 6.5 OF FERREDOXIN I FROM AZOTOBACTER VINELANDII AT 2.3 ANGSTROMS RESOLUTION

PDB ID 1fer

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