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1fg2

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(New page: 200px<br /><applet load="1fg2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fg2, resolution 2.754&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:1fg2.gif|left|200px]]<br /><applet load="1fg2" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fg2, resolution 2.754&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE LCMV PEPTIDIC EPITOPE GP33 IN COMPLEX WITH THE MURINE CLASS I MHC MOLECULE H-2DB'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE LCMV PEPTIDIC EPITOPE GP33 IN COMPLEX WITH THE MURINE CLASS I MHC MOLECULE H-2DB==
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Viral escape, first characterized for the lymphocytic choriomeningitis, virus (LCMV) in a mouse transgenic for the P14 T cell-receptor (TCR), can, be due to mutations in T-cell epitopes. We have measured the affinity, between the H-2D(b) containing the wild-type and two of its "viral escape", epitopes, as well as other altered peptide ligands (APL), by using BIACORE, analysis, and solved the crystal structure of H-2D(b) in complex with the, wild-type peptide at 2.75 A resolution. We show that viral escape is due, to a 50 to 100-fold reduction in the level of affinity between the P14 TCR, and the binary complexes of the MHC molecule with the different peptides., Structurally, one of the mutations alters a TCR contact residue, while the, effect of the other on the binding of the TCR must be indirect through, structural rearrangements. The former is a null ligand, while the latter, still leads to some central tolerance. This work defines the structural, and energetic threshold for viral escape.
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<StructureSection load='1fg2' size='340' side='right'caption='[[1fg2]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fg2]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FG2 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fg2 OCA], [https://pdbe.org/1fg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fg2 RCSB], [https://www.ebi.ac.uk/pdbsum/1fg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fg2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HA11_MOUSE HA11_MOUSE] Involved in the presentation of foreign antigens to the immune system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fg/1fg2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fg2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Viral escape, first characterized for the lymphocytic choriomeningitis virus (LCMV) in a mouse transgenic for the P14 T cell-receptor (TCR), can be due to mutations in T-cell epitopes. We have measured the affinity between the H-2D(b) containing the wild-type and two of its "viral escape" epitopes, as well as other altered peptide ligands (APL), by using BIACORE analysis, and solved the crystal structure of H-2D(b) in complex with the wild-type peptide at 2.75 A resolution. We show that viral escape is due to a 50 to 100-fold reduction in the level of affinity between the P14 TCR and the binary complexes of the MHC molecule with the different peptides. Structurally, one of the mutations alters a TCR contact residue, while the effect of the other on the binding of the TCR must be indirect through structural rearrangements. The former is a null ligand, while the latter still leads to some central tolerance. This work defines the structural and energetic threshold for viral escape.
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==About this Structure==
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Viral escape at the molecular level explained by quantitative T-cell receptor/peptide/MHC interactions and the crystal structure of a peptide/MHC complex.,Tissot AC, Ciatto C, Mittl PR, Grutter MG, Pluckthun A J Mol Biol. 2000 Sep 29;302(4):873-85. PMID:10993729<ref>PMID:10993729</ref>
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1FG2 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FG2 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Viral escape at the molecular level explained by quantitative T-cell receptor/peptide/MHC interactions and the crystal structure of a peptide/MHC complex., Tissot AC, Ciatto C, Mittl PR, Grutter MG, Pluckthun A, J Mol Biol. 2000 Sep 29;302(4):873-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10993729 10993729]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1fg2" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Ciatto, C.]]
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[[Category: Gruetter, M.G.]]
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[[Category: Mittl, P.R.E.]]
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[[Category: Plueckthun, A.]]
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[[Category: Tissot, A.C.]]
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[[Category: ig fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:53:58 2007''
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==See Also==
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*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
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*[[MHC 3D structures|MHC 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Ciatto C]]
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[[Category: Gruetter MG]]
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[[Category: Mittl PRE]]
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[[Category: Plueckthun A]]
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[[Category: Tissot AC]]

Current revision

CRYSTAL STRUCTURE OF THE LCMV PEPTIDIC EPITOPE GP33 IN COMPLEX WITH THE MURINE CLASS I MHC MOLECULE H-2DB

PDB ID 1fg2

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