1erj

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{{Seed}}
 
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[[Image:1erj.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1==
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The line below this paragraph, containing "STRUCTURE_1erj", creates the "Structure Box" on the page.
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<StructureSection load='1erj' size='340' side='right'caption='[[1erj]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1erj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ERJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1erj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1erj OCA], [https://pdbe.org/1erj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1erj RCSB], [https://www.ebi.ac.uk/pdbsum/1erj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1erj ProSAT]</span></td></tr>
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{{STRUCTURE_1erj| PDB=1erj | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TUP1_YEAST TUP1_YEAST] Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex.<ref>PMID:2247069</ref> <ref>PMID:11069890</ref> <ref>PMID:10722672</ref> <ref>PMID:11230135</ref> <ref>PMID:11172717</ref> <ref>PMID:11784848</ref> <ref>PMID:14665463</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/1erj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1erj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Tup1-Ssn6 corepressor complex regulates the expression of several sets of genes, including genes that specify mating type in the yeast Saccharomyces cerevisiae. Repression of mating-type genes occurs when Tup1-Ssn6 is brought to the DNA by the Matalpha2 DNA-binding protein and assembled upstream of a- and haploid-specific genes. We have determined the 2.3 A X-ray crystal structure of the C-terminal domain of Tup1 (accesion No. 1ERJ), a 43 kDa fragment that contains seven copies of the WD40 sequence motif and binds to the Matalpha2 protein. Moreover, this portion of the protein can partially substitute for full-length Tup1 in bringing about transcriptional repression. The structure reveals a seven-bladed beta propeller with an N-terminal subdomain that is anchored to the side of the propeller and extends the beta sheet of one of the blades. Point mutations in Tup1 that specifically affect the Tup1-Matalpha2 interaction cluster on one surface of the propeller. We identified regions of Tup1 that are conserved among the fungal Tup1 homologs and may be important in protein-protein interactions with additional components of the Tup1-mediated repression pathways.
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===CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1===
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Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast.,Sprague ER, Redd MJ, Johnson AD, Wolberger C EMBO J. 2000 Jun 15;19(12):3016-27. PMID:10856245<ref>PMID:10856245</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10856245}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1erj" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10856245 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10856245}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1ERJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ERJ OCA].
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==Reference==
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Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast., Sprague ER, Redd MJ, Johnson AD, Wolberger C, EMBO J. 2000 Jun 15;19(12):3016-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10856245 10856245]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Johnson AD]]
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[[Category: Johnson, A D.]]
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[[Category: Redd MJ]]
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[[Category: Redd, M J.]]
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[[Category: Sprague ER]]
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[[Category: Sprague, E R.]]
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[[Category: Wolberger C]]
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[[Category: Wolberger, C.]]
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[[Category: Beta-propeller]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:45:58 2008''
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Current revision

CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1

PDB ID 1erj

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