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1fhe

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(New page: 200px<br /><applet load="1fhe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fhe, resolution 3.&Aring;" /> '''GLUTATHIONE TRANSFERAS...)
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[[Image:1fhe.gif|left|200px]]<br /><applet load="1fhe" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fhe, resolution 3.&Aring;" />
 
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'''GLUTATHIONE TRANSFERASE (FH47) FROM FASCIOLA HEPATICA'''<br />
 
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==Overview==
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==GLUTATHIONE TRANSFERASE (FH47) FROM FASCIOLA HEPATICA==
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Glutathione S-transferases (GSTs) represent the major class of detoxifying, enzymes from parasitic helminths. As a result, they are candidates for, chemotherapeutic and vaccine design. Indeed, GSTs from Fasciola hepatica, have been found to be effective for vaccinating sheep and cattle against, fasciolosis. This helminth contains at least seven GST isoforms, of which, four have been cloned. The cloned isoforms (Fh51, Fh47, Fh7 and Fh1) all, belong to the mu class of GSTs, share greater than 71% sequence identity, yet display distinct substrate specificities. Crystals of Fh47 were, obtained using the hanging drop vapour diffusion technique. The crystals, belong to space group I4122, with one monomer in the asymmetric unit, which corresponds to a very high solvent content of approximately 75%. The, physiological dimer is generated via a crystallographic 2-fold rotation., The three-dimensional structure of Fh47 was solved by molecular, replacement using the Schistosoma japonicum glutathione S-transferase, (Sj26) crystal structure as a search model. The structure adopts the, canonical GST fold comprising two domains: an N-terminal, glutathione-binding domain, consisting of a four-stranded beta-sheet and, three helices whilst the C-terminal domain is entirely alpha-helical. The, presence of Phe19 in Fh47 results in a 6 degrees interdomain rotation in, comparison to Sj26, where the equivalent residue is a leucine. Homology, models of Fh51, Fh7 and Fh1, based on the Fh47 crystal structure, reveal, critical differences in the residues lining the xenobiotic binding site, particularly at residue positions 9, 106 and 204. In addition, differences, amongst the isoforms in the non-substrate binding site were noted, which, may explain the observed differential binding of large ligands. The major, immunogenic epitopes of Fh47 were surprisingly found not to reside on the, most solvent-exposed regions of the molecule.Copyright 1997 Academic Press, Limited
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<StructureSection load='1fhe' size='340' side='right'caption='[[1fhe]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fhe]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fasciola_hepatica Fasciola hepatica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FHE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhe OCA], [https://pdbe.org/1fhe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fhe RCSB], [https://www.ebi.ac.uk/pdbsum/1fhe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fhe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GST27_FASHE GST27_FASHE] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/1fhe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fhe ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FHE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fasciola_hepatica Fasciola hepatica] with GSW as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FHE OCA].
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*[[Glutathione S-transferase|Glutathione S-transferase]]
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*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
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==Reference==
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__TOC__
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Crystallization, structural determination and analysis of a novel parasite vaccine candidate: Fasciola hepatica glutathione S-transferase., Rossjohn J, Feil SC, Wilce MC, Sexton JL, Spithill TW, Parker MW, J Mol Biol. 1997 Nov 7;273(4):857-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9367777 9367777]
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</StructureSection>
[[Category: Fasciola hepatica]]
[[Category: Fasciola hepatica]]
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[[Category: Glutathione transferase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Parker MW]]
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[[Category: Parker, M.W.]]
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[[Category: Rossjohn J]]
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[[Category: Rossjohn, J.]]
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[[Category: GSW]]
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[[Category: detoxification]]
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[[Category: glutathione transferase]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:55:28 2007''
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Current revision

GLUTATHIONE TRANSFERASE (FH47) FROM FASCIOLA HEPATICA

PDB ID 1fhe

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