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| - | {{Seed}} | |
| - | [[Image:1ev7.png|left|200px]] | |
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| - | <!-- | + | ==CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI== |
| - | The line below this paragraph, containing "STRUCTURE_1ev7", creates the "Structure Box" on the page.
| + | <StructureSection load='1ev7' size='340' side='right'caption='[[1ev7]], [[Resolution|resolution]] 2.38Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1ev7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EV7 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38Å</td></tr> |
| - | --> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ev7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev7 OCA], [https://pdbe.org/1ev7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ev7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ev7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ev7 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1ev7| PDB=1ev7 | SCENE= }}
| + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/T2N1_LENAE T2N1_LENAE] An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.<ref>PMID:7892605</ref> <ref>PMID:8932368</ref> <ref>PMID:12654995</ref> |
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| - | ===CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI=== | + | ==See Also== |
| - | | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| - | | + | == References == |
| - | <!--
| + | <references/> |
| - | The line below this paragraph, {{ABSTRACT_PUBMED_10856254}}, adds the Publication Abstract to the page
| + | __TOC__ |
| - | (as it appears on PubMed at http://www.pubmed.gov), where 10856254 is the PubMed ID number.
| + | </StructureSection> |
| - | -->
| + | [[Category: Large Structures]] |
| - | {{ABSTRACT_PUBMED_10856254}}
| + | [[Category: Lentzea aerocolonigenes]] |
| - | | + | [[Category: Chen Y]] |
| - | ==About this Structure==
| + | [[Category: Colandene JD]] |
| - | 1EV7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA].
| + | [[Category: Huai Q]] |
| - | | + | [[Category: Luo F]] |
| - | ==Reference== | + | [[Category: Zhao Y]] |
| - | Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase., Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H, EMBO J. 2000 Jun 15;19(12):3110-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10856254 10856254]
| + | |
| - | [[Category: Lechevalieria aerocolonigenes]] | + | |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Type II site-specific deoxyribonuclease]]
| + | |
| - | [[Category: Chen, Y.]] | + | |
| - | [[Category: Colandene, J D.]] | + | |
| - | [[Category: Huai, Q.]] | + | |
| - | [[Category: Luo, F.]] | + | |
| - | [[Category: Zhao, Y.]] | + | |
| - | [[Category: Apo-naei]]
| + | |
| - | [[Category: Cap]]
| + | |
| - | [[Category: Helix-turn-helix]]
| + | |
| - | [[Category: Restriction endonuclease]]
| + | |
| - | [[Category: Topoisomerase]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:04:25 2008''
| + | |
| Structural highlights
Function
T2N1_LENAE An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.[1] [2] [3]
See Also
References
- ↑ Jo K, Topal MD. DNA topoisomerase and recombinase activities in Nae I restriction endonuclease. Science. 1995 Mar 24;267(5205):1817-20. doi: 10.1126/science.7892605. PMID:7892605 doi:http://dx.doi.org/10.1126/science.7892605
- ↑ Jo K, Topal MD. Effects on NaeI-DNA recognition of the leucine to lysine substitution that transforms restriction endonuclease NaeI to a topoisomerase: a model for restriction endonuclease evolution. Nucleic Acids Res. 1996 Nov 1;24(21):4171-5. doi: 10.1093/nar/24.21.4171. PMID:8932368 doi:http://dx.doi.org/10.1093/nar/24.21.4171
- ↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SKh, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Kruger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. 2003 Apr 1;31(7):1805-12. PMID:12654995
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