1ezk

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{{Seed}}
 
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[[Image:1ezk.png|left|200px]]
 
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==Crystal structure of recombinant tryparedoxin I==
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The line below this paragraph, containing "STRUCTURE_1ezk", creates the "Structure Box" on the page.
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<StructureSection load='1ezk' size='340' side='right'caption='[[1ezk]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ezk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EZK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ezk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ezk OCA], [https://pdbe.org/1ezk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ezk RCSB], [https://www.ebi.ac.uk/pdbsum/1ezk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ezk ProSAT]</span></td></tr>
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{{STRUCTURE_1ezk| PDB=1ezk | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O96438_CRIFA O96438_CRIFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/1ezk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ezk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tryparedoxins (TXNs) catalyse the reduction of peroxiredoxin-type peroxidases by the bis-glutathionyl derivative of spermidine, trypanothione, and are relevant to hydroperoxide detoxification and virulence of trypanosomes. The 3D-structures of the following tryparedoxins are presented: authentic tryparedoxin1 of Crithidia fasciculata, CfTXN1; the his-tagged recombinant protein, CfTXN1H6; reduced and oxidised CfTXN2, and an alternative substrate derivative of the mutein CfTXN2H6-Cys44Ser. Cys41 (Cys40 in TXN1) of the active site motif 40-WCPPCR-45 proved to be the only solvent-exposed redox active residue in CfTXN2. In reduced TXNs, its nucleophilicity is increased by a network of hydrogen bonds. In oxidised TXNs it can be attacked by the thiol of the 1N-glutathionyl residue of trypanothione, as evidenced by the structure of 1N-glutathionylspermidine-derivatised CfTXN2H6-Cys44Ser. Modelling suggests Arg45 (44), Glu73 (72), the Ile110 (109) cis-Pro111 (110)-bond and Arg129 (128) to be involved in the binding of trypanothione to CfTXN2 (CfTXN1). The model of TXN-substrate interaction is consistent with functional characteristics of known and newly designed muteins (CfTXN2H6-Arg129Asp and Glu73Arg) and the 1N-glutathionyl-spermidine binding in the CfTXN2H6-Cys44Ser structure.
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===Crystal structure of recombinant tryparedoxin I===
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Structures of tryparedoxins revealing interaction with trypanothione.,Hofmann B, Budde H, Bruns K, Guerrero SA, Kalisz HM, Menge U, Montemartini M, Nogoceke E, Steinert P, Wissing JB, Flohe L, Hecht HJ Biol Chem. 2001 Mar;382(3):459-71. PMID:11347894<ref>PMID:11347894</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_11347894}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1ezk" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11347894 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11347894}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1EZK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Crithidia_fasciculata Crithidia fasciculata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZK OCA].
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==Reference==
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Structures of tryparedoxins revealing interaction with trypanothione., Hofmann B, Budde H, Bruns K, Guerrero SA, Kalisz HM, Menge U, Montemartini M, Nogoceke E, Steinert P, Wissing JB, Flohe L, Hecht HJ, Biol Chem. 2001 Mar;382(3):459-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11347894 11347894]
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[[Category: Crithidia fasciculata]]
[[Category: Crithidia fasciculata]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Flohe, L.]]
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[[Category: Flohe L]]
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[[Category: Guerrero, S A.]]
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[[Category: Guerrero SA]]
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[[Category: Hecht, H J.]]
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[[Category: Hecht HJ]]
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[[Category: Hofmann, B.]]
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[[Category: Hofmann B]]
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[[Category: Kalisz, H M.]]
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[[Category: Kalisz HM]]
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[[Category: Menge, U.]]
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[[Category: Menge U]]
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[[Category: Montemartini, M.]]
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[[Category: Montemartini M]]
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[[Category: Nogoceke, E.]]
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[[Category: Nogoceke E]]
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[[Category: Singh, M.]]
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[[Category: Singh M]]
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[[Category: Electron transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:15:22 2008''
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Current revision

Crystal structure of recombinant tryparedoxin I

PDB ID 1ezk

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