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1flm
From Proteopedia
(Difference between revisions)
(New page: 200px<br /><applet load="1flm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1flm, resolution 1.30Å" /> '''DIMER OF FMN-BINDING...) |
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| - | [[Image:1flm.gif|left|200px]]<br /><applet load="1flm" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1flm, resolution 1.30Å" /> | ||
| - | '''DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)'''<br /> | ||
| - | == | + | ==DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)== |
| - | The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio | + | <StructureSection load='1flm' size='340' side='right'caption='[[1flm]], [[Resolution|resolution]] 1.30Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1flm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._'Miyazaki_F' Desulfovibrio vulgaris str. 'Miyazaki F']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FLM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FLM FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1flm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1flm OCA], [https://pdbe.org/1flm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1flm RCSB], [https://www.ebi.ac.uk/pdbsum/1flm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1flm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/FMNB_DESVM FMNB_DESVM] Functions as a redox protein with a potential of -325 mV. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/1flm_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1flm ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of FMN-binding protein (FMN-bp) from Desulfovibrio vulgaris Miyazaki F was solved by the multiple isomorphous replacement method and refined to an R factor of 15.1% at 1.3 A resolution. FMN-bp exists in a dimeric form in the crystal, in contrast to the monomeric structure determined by NMR. R.m.s. deviations between the crystal structure and the solution structure are more than 2 A, which implies significant differences. There are some hydrophobic residues in the interface between the two monomers. In particular, Leu122 in the C-terminus has a close contact with the o-xylene moiety of FMN, while solvent molecules may cover the o-xylene moiety in the solution structure. | ||
| - | + | How do the x-ray structure and the NMR structure of FMN-binding protein differ?,Suto K, Kawagoe K, Shibata N, Morimoto Y, Higuchi Y, Kitamura M, Nakaya T, Yasuoka N Acta Crystallogr D Biol Crystallogr. 2000 Mar;56(Pt 3):368-71. PMID:10713530<ref>PMID:10713530</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Desulfovibrio vulgaris]] | + | <div class="pdbe-citations 1flm" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Higuchi | + | <references/> |
| - | [[Category: Kawagoe | + | __TOC__ |
| - | [[Category: Kitamura | + | </StructureSection> |
| - | [[Category: Morimoto | + | [[Category: Desulfovibrio vulgaris str. 'Miyazaki F']] |
| - | [[Category: Nakaya | + | [[Category: Large Structures]] |
| - | [[Category: Shibata | + | [[Category: Higuchi Y]] |
| - | [[Category: Suto | + | [[Category: Kawagoe K]] |
| - | [[Category: Yasuoka | + | [[Category: Kitamura M]] |
| - | + | [[Category: Morimoto K]] | |
| - | + | [[Category: Nakaya T]] | |
| - | + | [[Category: Shibata N]] | |
| - | + | [[Category: Suto K]] | |
| + | [[Category: Yasuoka N]] | ||
Current revision
DIMER OF FMN-BINDING PROTEIN FROM DESULFOVIBRIO VULGARIS (MIYAZAKI F)
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