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1fps

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(New page: 200px<br /><applet load="1fps" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fps, resolution 2.6&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1fps.jpg|left|200px]]<br /><applet load="1fps" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fps, resolution 2.6&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RECOMBINANT FARNESYL DIPHOSPHATE SYNTHASE AT 2.6 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF RECOMBINANT FARNESYL DIPHOSPHATE SYNTHASE AT 2.6 ANGSTROMS RESOLUTION==
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The synthesis of farnesyl diphosphate (FPP), a key intermediate in the, isoprenoid biosynthetic pathway required for the synthesis of cholesterol, and in the formation of prenylated proteins, is catalyzed by the enzyme, farnesyl diphosphate synthase (FPS). The crystal structure of avian, recombinant FPS, the first three-dimensional structure for any, prenyltransferase, was determined to 2.6-A resolution. The enzyme exhibits, a novel fold composed entirely of alpha-helices joined by connecting, loops. The enzyme's most prominent structural feature is the arrangement, of 10 core helices around a large central cavity. Two aspartate-rich, sequences that are highly conserved among the isoprenyl diphosphate, synthase family of prenyltransferases, and are essential for enzymatic, activity, were found on opposite walls of this cavity, with the aspartate, side chains approximately 12 A apart and facing each other. The location, and metal ion binding properties of these sequences suggest that the, conserved aspartate residues participate in substrate binding of, catalysis.
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<StructureSection load='1fps' size='340' side='right'caption='[[1fps]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fps]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FPS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FPS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fps FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fps OCA], [https://pdbe.org/1fps PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fps RCSB], [https://www.ebi.ac.uk/pdbsum/1fps PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fps ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FPPS_CHICK FPPS_CHICK] Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fp/1fps_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fps ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FPS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Active as [http://en.wikipedia.org/wiki/Geranyltranstransferase Geranyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.10 2.5.1.10] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FPS OCA].
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*[[Farnesyl diphosphate synthase 3D structures|Farnesyl diphosphate synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of recombinant farnesyl diphosphate synthase at 2.6-A resolution., Tarshis LC, Yan M, Poulter CD, Sacchettini JC, Biochemistry. 1994 Sep 13;33(36):10871-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8086404 8086404]
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[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Geranyltranstransferase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Poulter CD]]
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[[Category: Poulter, C.D.]]
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[[Category: Sacchettini JC]]
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[[Category: Sacchettini, J.C.]]
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[[Category: Tarshis LC]]
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[[Category: Tarshis, L.C.]]
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[[Category: Yan M]]
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[[Category: Yan, M.]]
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[[Category: prenyltransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:08:07 2007''
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Current revision

CRYSTAL STRUCTURE OF RECOMBINANT FARNESYL DIPHOSPHATE SYNTHASE AT 2.6 ANGSTROMS RESOLUTION

PDB ID 1fps

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