8cho

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="8cho" size="450" color="white" frame="true" align="right" spinBox="true" caption="8cho, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
Current revision (09:40, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:8cho.gif|left|200px]]<br /><applet load="8cho" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="8cho, resolution 2.3&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF DELTA5-3-KETOSTEROID ISOMERASE FROM PSEUDOMONAS TESTOSTERONI'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF DELTA5-3-KETOSTEROID ISOMERASE FROM PSEUDOMONAS TESTOSTERONI==
-
Bacterial Delta 5-3-ketosteroid isomerase (KSI) from Pseudomonas, testosteroni has been intensively studied as a prototype for understanding, an enzyme-catalyzed allylic rearrangement involving intramolecular proton, transfer. Asp38 serves as a general base to abstract the proton from the, steroid C4-H, which is a much stronger base than the carboxyl group of, this residue. This unfavorable proton transfer requires 11 kcal/mol of, energy which has to be provided by favorable interactions between, catalytic residues and substrate in the course of the catalytic reaction., How this energy is provided at the active site of KSI has been a, controversial issue, and inevitably the enzyme mechanism is not settled., To resolve these issues, we have determined the crystal structure of this, enzyme at 2.3 A resolution. The crystal structure revealed that the active, site environment of P. testosteroni KSI is nearly identical to that of, Pseudomonas putida KSI, whose structure in complex with a reaction, intermediate analogue we have determined recently. Comparison of the two, structures clearly indicates that the two KSIs should share the same, enzyme mechanism involving the stabilization of the dienolate intermediate, by the two direct hydrogen bonds to the dienolate oxyanion, one from Tyr14, OH and the other from Asp99 COOH. Mutational analysis of the two residues, and other biochemical data strongly suggest that the hydrogen bond of, Tyr14 provides the more significant contribution than that of Asp99 to the, requisite 11 kcal/mol of energy for the catalytic power of KSI.
+
<StructureSection load='8cho' size='340' side='right'caption='[[8cho]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[8cho]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8CHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8CHO FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P4C:O-ACETALDEHYDYL-HEXAETHYLENE+GLYCOL'>P4C</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8cho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8cho OCA], [https://pdbe.org/8cho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8cho RCSB], [https://www.ebi.ac.uk/pdbsum/8cho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8cho ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SDIS_COMTE SDIS_COMTE]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/8cho_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=8cho ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
8CHO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Comamonas_testosteroni Comamonas testosteroni] with P4C as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=8CHO OCA].
+
*[[Ketosteroid Isomerase|Ketosteroid Isomerase]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure and enzyme mechanism of Delta 5-3-ketosteroid isomerase from Pseudomonas testosteroni., Cho HS, Choi G, Choi KY, Oh BH, Biochemistry. 1998 Jun 9;37(23):8325-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9622484 9622484]
+
[[Category: Comamonas testosteroni]]
[[Category: Comamonas testosteroni]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Steroid Delta-isomerase]]
+
[[Category: Cho H-S]]
-
[[Category: Cho, H.S.]]
+
[[Category: Oh B-H]]
-
[[Category: Oh, B.H.]]
+
-
[[Category: P4C]]
+
-
[[Category: isomerase]]
+
-
[[Category: steroid isomeration]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:10:11 2007''
+

Current revision

CRYSTAL STRUCTURE OF DELTA5-3-KETOSTEROID ISOMERASE FROM PSEUDOMONAS TESTOSTERONI

PDB ID 8cho

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8cho"
Personal tools