This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1frm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1frm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1frm, resolution 2.3&Aring;" /> '''AZOTOBACTER VINELANDI...)
Current revision (07:18, 7 February 2024) (edit) (undo)
 
(19 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1frm.gif|left|200px]]<br /><applet load="1frm" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1frm, resolution 2.3&Aring;" />
 
-
'''AZOTOBACTER VINELANDII FERREDOXIN I: ALTERATION OF INDIVIDUAL SURFACE CHARGES AND THE [4FE-4S] CLUSTER REDUCTION POTENTIAL'''<br />
 
-
==Overview==
+
==AZOTOBACTER VINELANDII FERREDOXIN I: ALTERATION OF INDIVIDUAL SURFACE CHARGES AND THE [4FE-4S] CLUSTER REDUCTION POTENTIAL==
-
The structures of Azotobacter vinelandii ferredoxin I (AvFdI) and, Peptococcus aerogenes ferredoxin (PaFd), near their analogous [4e-4S]2+/+, clusters, are highly conserved (Backes, G., Mino, Y., Loehr, T.M., Meyer, T.E., Cusanovich, M.A., Sweeney, W.V., Adman, E.T., and Sanders-Loehr, J., (1991) J. Am. Chem. Soc. 11, 2055-2064). Despite these similarities, the, reduction potential (E0') of the AvFdI [4Fe-4S]2+/+ cluster is more than, 200 mV more negative than that of PaFd. We have tested the contribution, that individual amino acid residues make to the control of E0' by, converting residues in AvFdI into the corresponding residue in PaFd. Four, mutations involved substitutions of negatively charged surface residues, with neutral residues and two involved substitution of buried hydrophobic, residues. All AvFdI variants were characterized by x-ray crystallography, absorption, CD, EPR, and 1H NMR spectroscopies and by electrochemical, methods. For the F25I mutation, significant structural changes occurred, that affected the EPR and 1H NMR spectroscopic properties of AvFdI and had, a minor influence on E0'. For all other mutations there were no changes in, reduction potential. Thus we conclude, that variations in charged surface, residues do not account for the observed differences in E0' between the, analogous [4Fe-4S]2+/+ cluster of PaFd and AvFdI. These differences are, therefore most likely to be due to differences in solvent accessibility.
+
<StructureSection load='1frm' size='340' side='right'caption='[[1frm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1frm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FRM FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1frm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1frm OCA], [https://pdbe.org/1frm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1frm RCSB], [https://www.ebi.ac.uk/pdbsum/1frm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1frm ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/FER1_AZOVI FER1_AZOVI] Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions. This ferredoxin could play a role in regulating gene expression by interacting directly with DNA.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fr/1frm_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1frm ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1FRM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with SF4 and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FRM OCA].
+
*[[Ferredoxin 3D structures|Ferredoxin 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Azotobacter vinelandii ferredoxin I. Alteration of individual surface charges and the [4FE-4S]2+/+ cluster reduction potential., Shen B, Jollie DR, Stout CD, Diller TC, Armstrong FA, Gorst CM, La Mar GN, Stephens PJ, Burgess BK, J Biol Chem. 1994 Mar 18;269(11):8564-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8132582 8132582]
+
[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Stout, C.D.]]
+
[[Category: Stout CD]]
-
[[Category: F3S]]
+
-
[[Category: SF4]]
+
-
[[Category: electron transport]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:12:03 2007''
+

Current revision

AZOTOBACTER VINELANDII FERREDOXIN I: ALTERATION OF INDIVIDUAL SURFACE CHARGES AND THE [4FE-4S] CLUSTER REDUCTION POTENTIAL

PDB ID 1frm

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1frm"
Personal tools