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7pcy

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(New page: 200px<br /><applet load="7pcy" size="450" color="white" frame="true" align="right" spinBox="true" caption="7pcy, resolution 1.8&Aring;" /> '''THE CRYSTAL STRUCTURE...)
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[[Image:7pcy.gif|left|200px]]<br /><applet load="7pcy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="7pcy, resolution 1.8&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF PLASTOCYANIN FROM A GREEN ALGA, ENTEROMORPHA PROLIFERA'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF PLASTOCYANIN FROM A GREEN ALGA, ENTEROMORPHA PROLIFERA==
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The crystal structure of the Cu-containing protein plastocyanin (Mr, 10,500) from the green alga Enteromorpha prolifera has been solved by, molecular replacement. The structure was refined by constrained-restrained, and restrained reciprocal space least-squares techniques. The refined, model includes 111 solvent sites. There is evidence for alternate, conformers at eight residues. The residual is 0.12 for a data set, comprising 74% of all observations accessible at 1.85 A resolution. The, beta-sandwich structure of the algal plastocyanin is effectively the same, as that of poplar leaf (Populus nigra var. italica) plastocyanin, determined at 1.6 A resolution. The sequence homology between the two, proteins is 56%. Differences between the contacts in the hydrophobic core, create some significant (0.5 to 1.2 A) movements of the polypeptide, backbone, resulting in small differences between the orientations and, separations of corresponding beta-strands. These differences are most, pronounced at the end of the molecule remote from the Cu site. The largest, structural differences occur in the single non-beta strand, which includes, the sole turn of helix in the molecule: two of the residues in a prominent, kink of the poplar plastocyanin backbone are missing from the algal, plastocyanin sequence, and there is a significant change in the position, of the helical segment in relation to the beta-sandwich. Several other, small but significant structural differences can be correlated with, intermolecular contacts in the crystals. An intramolecular, carboxyl-carboxylate hydrogen bond in the algal plastocyanin may be, associated with an unusually high pKa. The dimensions of the Cu site in, the two plastocyanins are, within the limits of precision, identical.
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<StructureSection load='7pcy' size='340' side='right'caption='[[7pcy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7pcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ulva_prolifera Ulva prolifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PCY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pcy OCA], [https://pdbe.org/7pcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pcy RCSB], [https://www.ebi.ac.uk/pdbsum/7pcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pcy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLAS_ULVPR PLAS_ULVPR] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/7pcy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=7pcy ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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7PCY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ulva_prolifera Ulva prolifera] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=7PCY OCA].
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*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of plastocyanin from a green alga, Enteromorpha prolifera., Collyer CA, Guss JM, Sugimura Y, Yoshizaki F, Freeman HC, J Mol Biol. 1990 Feb 5;211(3):617-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2308169 2308169]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Ulva prolifera]]
[[Category: Ulva prolifera]]
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[[Category: Collyer, C.A.]]
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[[Category: Collyer CA]]
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[[Category: Freeman, H.C.]]
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[[Category: Freeman HC]]
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[[Category: Guss, J.M.]]
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[[Category: Guss JM]]
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[[Category: CU]]
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[[Category: electron transport protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:13:30 2007''
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Current revision

THE CRYSTAL STRUCTURE OF PLASTOCYANIN FROM A GREEN ALGA, ENTEROMORPHA PROLIFERA

PDB ID 7pcy

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