1fs8

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(New page: 200px<br /><applet load="1fs8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fs8, resolution 1.60&Aring;" /> '''CYTOCHROME C NITRITE...)
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[[Image:1fs8.jpg|left|200px]]<br /><applet load="1fs8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fs8, resolution 1.60&Aring;" />
 
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'''CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES-SULFATE COMPLEX'''<br />
 
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==Overview==
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==CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES-SULFATE COMPLEX==
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Cytochrome c nitrite reductase catalyzes the 6-electron reduction of, nitrite to ammonia. This second part of the respiratory pathway of nitrate, ammonification is a key step in the biological nitrogen cycle. The x-ray, structure of the enzyme from the epsilon-proteobacterium Wolinella, succinogenes has been solved to a resolution of 1.6 A. It is a pentaheme, c-type cytochrome whose heme groups are packed in characteristic motifs, that also occur in other multiheme cytochromes. Structures of W., succinogenes nitrite reductase have been obtained with water bound to the, active site heme iron as well as complexes with two inhibitors, sulfate, and azide, whose binding modes and inhibitory functions differ, significantly. Cytochrome c nitrite reductase is part of a highly, optimized respiratory system found in a wide range of Gram-negative, bacteria. It reduces both anionic and neutral substrates at the distal, side of a lysine-coordinated high-spin heme group, which is accessible, through two different channels, allowing for a guided flow of reaction, educt and product. Based on sequence comparison and secondary structure, prediction, we have demonstrated that cytochrome c nitrite reductases, constitute a protein family of high structural similarity.
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<StructureSection load='1fs8' size='340' side='right'caption='[[1fs8]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fs8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FS8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FS8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=Y1:YTTRIUM+ION'>Y1</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fs8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fs8 OCA], [https://pdbe.org/1fs8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fs8 RCSB], [https://www.ebi.ac.uk/pdbsum/1fs8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fs8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NRFA_WOLSU NRFA_WOLSU] Plays a role in nitrite reduction.<ref>PMID:10672190</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/1fs8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fs8 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FS8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes] with CA, Y1, SO4, ACT and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FS8 OCA].
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*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
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*[[Cytochrome c nitrite reductase|Cytochrome c nitrite reductase]]
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==Reference==
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== References ==
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Cytochrome c nitrite reductase from Wolinella succinogenes. Structure at 1.6 A resolution, inhibitor binding, and heme-packing motifs., Einsle O, Stach P, Messerschmidt A, Simon J, Kroger A, Huber R, Kroneck PM, J Biol Chem. 2000 Dec 15;275(50):39608-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10984487 10984487]
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<references/>
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[[Category: Single protein]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Wolinella succinogenes]]
[[Category: Wolinella succinogenes]]
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[[Category: Einsle, O.]]
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[[Category: Einsle O]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: Kroeger, A.]]
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[[Category: Kroeger A]]
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[[Category: Kroneck, P.M.H.]]
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[[Category: Kroneck PMH]]
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[[Category: Messerschmidt, A.]]
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[[Category: Messerschmidt A]]
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[[Category: Simon, J.]]
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[[Category: Simon J]]
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[[Category: Stach, P.]]
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[[Category: Stach P]]
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[[Category: ACT]]
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[[Category: CA]]
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[[Category: HEM]]
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[[Category: SO4]]
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[[Category: Y1]]
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[[Category: c-type cytochrome]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:13:54 2007''
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Current revision

CYTOCHROME C NITRITE REDUCTASE FROM WOLINELLA SUCCINOGENES-SULFATE COMPLEX

PDB ID 1fs8

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