1fuk

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(New page: 200px<br /><applet load="1fuk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fuk, resolution 1.75&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1fuk.jpg|left|200px]]<br /><applet load="1fuk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fuk, resolution 1.75&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE CARBOXY TERMINAL DOMAIN OF YEAST EIF4A'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE CARBOXY TERMINAL DOMAIN OF YEAST EIF4A==
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The eukaryotic translation initiation factor 4A (eIF4A) is a member of the, DEA(D/H)-box RNA helicase family, a diverse group of proteins that couples, an ATPase activity to RNA binding and unwinding. Previous work has, provided the structure of the amino-terminal, ATP-binding domain of eIF4A., Extending those results, we have solved the structure of the, carboxyl-terminal domain of eIF4A with data to 1.75 A resolution; it has a, parallel alpha-beta topology that superimposes, with minor variations, on, the structures and conserved motifs of the equivalent domain in other, distantly related helicases. Using data to 2.8 A resolution and molecular, replacement with the refined model of the carboxyl-terminal domain, we, have completed the structure of full-length eIF4A; it is a "dumbbell", structure consisting of two compact domains connected by an extended, linker. By using the structures of other helicases as a template, compact, structures can be modeled for eIF4A that suggest (i) helicase motif IV, binds RNA; (ii) Arg-298, which is conserved in the DEA(D/H)-box RNA, helicase family but is absent from many other helicases, also binds RNA;, and (iii) motifs V and VI "link" the carboxyl-terminal domain to the, amino-terminal domain through interactions with ATP and the DEA(D/H), motif, providing a mechanism for coupling ATP binding and hydrolysis with, conformational changes that modulate RNA binding.
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<StructureSection load='1fuk' size='340' side='right'caption='[[1fuk]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FUK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fuk OCA], [https://pdbe.org/1fuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fuk RCSB], [https://www.ebi.ac.uk/pdbsum/1fuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fuk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IF4A_YEAST IF4A_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/1fuk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fuk ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FUK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FUK OCA].
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase., Caruthers JM, Johnson ER, McKay DB, Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13080-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11087862 11087862]
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Caruthers JM]]
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[[Category: Caruthers, J.M.]]
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[[Category: Johnson ER]]
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[[Category: Johnson, E.R.]]
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[[Category: McKay DB]]
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[[Category: McKay, D.B.]]
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[[Category: ZN]]
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[[Category: dead-box protein]]
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[[Category: helicase]]
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[[Category: initiation factor 4a]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:18:07 2007''
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Current revision

CRYSTAL STRUCTURE OF THE CARBOXY TERMINAL DOMAIN OF YEAST EIF4A

PDB ID 1fuk

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