6tmn

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(New page: 200px<br /><applet load="6tmn" size="450" color="white" frame="true" align="right" spinBox="true" caption="6tmn, resolution 1.6&Aring;" /> '''STRUCTURES OF TWO THE...)
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[[Image:6tmn.gif|left|200px]]<br /><applet load="6tmn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="6tmn, resolution 1.6&Aring;" />
 
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'''STRUCTURES OF TWO THERMOLYSIN-INHIBITOR COMPLEXES THAT DIFFER BY A SINGLE HYDROGEN BOND'''<br />
 
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==Overview==
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==Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond==
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The mode of binding to thermolysin of the ester analog, Cbz-GlyP-(O)-Leu-Leu has been determined by x-ray crystallography and, shown to be virtually identical (maximum difference 0.2 angstrom) with the, corresponding peptide analog Cbz-GlyP-(NH)-Leu-Leu. The two inhibitors, provide a matched pair of enzyme-inhibitor complexes that differ by 4.1, kilocalories per mole in intrinsic binding energy but are essentially, identical except for the presence or absence of a specific hydrogen bond.
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<StructureSection load='6tmn' size='340' side='right'caption='[[6tmn]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6tmn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_thermoproteolyticus Bacillus thermoproteolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TMN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0PI:N-[(2R,4S)-4-HYDROXY-2-(2-METHYLPROPYL)-4-OXIDO-7-OXO-9-PHENYL-3,8-DIOXA-6-AZA-4-PHOSPHANONAN-1-OYL]-L-LEUCINE'>0PI</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tmn OCA], [https://pdbe.org/6tmn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tmn RCSB], [https://www.ebi.ac.uk/pdbsum/6tmn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tmn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/THER_BACTH THER_BACTH] Extracellular zinc metalloprotease.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tm/6tmn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=6tmn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The mode of binding to thermolysin of the ester analog Cbz-GlyP-(O)-Leu-Leu has been determined by x-ray crystallography and shown to be virtually identical (maximum difference 0.2 angstrom) with the corresponding peptide analog Cbz-GlyP-(NH)-Leu-Leu. The two inhibitors provide a matched pair of enzyme-inhibitor complexes that differ by 4.1 kilocalories per mole in intrinsic binding energy but are essentially identical except for the presence or absence of a specific hydrogen bond.
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==About this Structure==
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Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond.,Tronrud DE, Holden HM, Matthews BW Science. 1987 Jan 30;235(4788):571-4. PMID:3810156<ref>PMID:3810156</ref>
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6TMN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with CA and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=6TMN OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond., Tronrud DE, Holden HM, Matthews BW, Science. 1987 Jan 30;235(4788):571-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3810156 3810156]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 6tmn" style="background-color:#fffaf0;"></div>
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[[Category: Thermolysin]]
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[[Category: Holden, H.M.]]
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[[Category: Matthews, B.W.]]
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[[Category: Tronrud, D.E.]]
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[[Category: CA]]
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[[Category: ZN]]
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[[Category: hydrolase (metalloproteinase)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:18:43 2007''
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==See Also==
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*[[Thermolysin 3D structures|Thermolysin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bacillus thermoproteolyticus]]
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[[Category: Large Structures]]
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[[Category: Holden HM]]
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[[Category: Matthews BW]]
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[[Category: Tronrud DE]]

Current revision

Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond

PDB ID 6tmn

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