1gk6

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{{Seed}}
 
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[[Image:1gk6.png|left|200px]]
 
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==Human vimentin coil 2B fragment linked to GCN4 leucine zipper (Z2B)==
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The line below this paragraph, containing "STRUCTURE_1gk6", creates the "Structure Box" on the page.
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<StructureSection load='1gk6' size='340' side='right'caption='[[1gk6]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1gk6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1e7t 1e7t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GK6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gk6 OCA], [https://pdbe.org/1gk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gk6 RCSB], [https://www.ebi.ac.uk/pdbsum/1gk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gk6 ProSAT]</span></td></tr>
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{{STRUCTURE_1gk6| PDB=1gk6 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VIME_HUMAN VIME_HUMAN] Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.<ref>PMID:21746880</ref> Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.<ref>PMID:21746880</ref> [https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/1gk6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gk6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Intermediate filaments (IFs) are key components of the cytoskeleton in higher eukaryotic cells. The elementary IF 'building block' is an elongated coiled-coil dimer consisting of four consecutive alpha-helical segments. The segments 1A and 2B include highly conserved sequences and are critically involved in IF assembly. Based on the crystal structures of three human vimentin fragments at 1.4-2.3 A resolution (PDB entries 1gk4, 1gk6 and 1gk7), we have established the molecular organization of these two segments. The fragment corresponding to segment 1A forms a single, amphipatic alpha-helix, which is compatible with a coiled-coil geometry. While this segment might yield a coiled coil within an isolated dimer, monomeric 1A helices are likely to play a role in specific dimer-dimer interactions during IF assembly. The 2B segment reveals a double-stranded coiled coil, which unwinds near residue Phe351 to accommodate a 'stutter'. A fragment containing the last seven heptads of 2B interferes heavily with IF assembly and also transforms mature vimentin filaments into a new kind of structure. These results provide the first insight into the architecture and functioning of IFs at the atomic level.
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===HUMAN VIMENTIN COIL 2B FRAGMENT LINKED TO GCN4 LEUCINE ZIPPER (Z2B)===
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Conserved segments 1A and 2B of the intermediate filament dimer: their atomic structures and role in filament assembly.,Strelkov SV, Herrmann H, Geisler N, Wedig T, Zimbelmann R, Aebi U, Burkhard P EMBO J. 2002 Mar 15;21(6):1255-66. PMID:11889032<ref>PMID:11889032</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1gk6" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11889032}}, adds the Publication Abstract to the page
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*[[Gcn4 3D Structures|Gcn4 3D Structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11889032 is the PubMed ID number.
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*[[Gnc4 3D Structures|Gnc4 3D Structures]]
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== References ==
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{{ABSTRACT_PUBMED_11889032}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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1GK6 is a [[Single protein]] structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1e7t 1e7t]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GK6 OCA].
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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==Reference==
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[[Category: Saccharomyces cerevisiae]]
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Conserved segments 1A and 2B of the intermediate filament dimer: their atomic structures and role in filament assembly., Strelkov SV, Herrmann H, Geisler N, Wedig T, Zimbelmann R, Aebi U, Burkhard P, EMBO J. 2002 Mar 15;21(6):1255-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11889032 11889032]
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[[Category: Aebi U]]
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[[Category: Single protein]]
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[[Category: Burkhard P]]
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[[Category: Aebi, U.]]
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[[Category: Geisler N]]
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[[Category: Burkhard, P.]]
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[[Category: Herrmann H]]
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[[Category: Geisler, N.]]
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[[Category: Strelkov SV]]
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[[Category: Herrmann, H.]]
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[[Category: Zimbelmann R]]
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[[Category: Strelkov, S V.]]
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[[Category: Zimbelmann, R.]]
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[[Category: Dimer]]
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[[Category: Fusion protein]]
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[[Category: Heptad repeat]]
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[[Category: Intermediate filament]]
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[[Category: Leucine zipper]]
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[[Category: Parallel coiled coil]]
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[[Category: Vimentin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:25:31 2008''
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Current revision

Human vimentin coil 2B fragment linked to GCN4 leucine zipper (Z2B)

PDB ID 1gk6

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