4gsa

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(New page: 200px<br /> <applet load="4gsa" size="450" color="white" frame="true" align="right" spinBox="true" caption="4gsa, resolution 2.50&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:4gsa.gif|left|200px]]<br />
 
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<applet load="4gsa" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="4gsa, resolution 2.50&Aring;" />
 
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'''CRYSTAL STRUCTURE OF GLUTAMATE-1-SEMIALDEHYDE AMINOMUTASE (AMINOTRANSFERASE) REDUCED WITH CYANOBOROHYDRATE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF GLUTAMATE-1-SEMIALDEHYDE AMINOMUTASE (AMINOTRANSFERASE) REDUCED WITH CYANOBOROHYDRATE==
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The three-dimensional structure of glutamate-1-semialdehyde aminomutase, (EC 5.4.3.8), an alpha2-dimeric enzyme from Synechococcus, has been, determined by x-ray crystallography using heavy atom derivative phasing., The structure, refined at 2.4-A resolution to an R-factor of 18.7% and, good stereochemistry, explains many of the enzyme's unusual specificity, and functional properties. The overall fold is that of aspartate, aminotransferase and related B6 enzymes, but it also has specific, features. The structure of the complex with gabaculine, a substrate, analogue, shows unexpectedly that the substrate binding site involves, residues from the N-terminal domain of the molecule, notably Arg-32., Glu-406 is suitably positioned to repel alpha-carboxylic acids, thereby, suggesting a basis for ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9144156 (full description)]]
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<StructureSection load='4gsa' size='340' side='right'caption='[[4gsa]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4gsa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GSA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GSA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gsa OCA], [https://pdbe.org/4gsa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gsa RCSB], [https://www.ebi.ac.uk/pdbsum/4gsa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gsa ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GSA_SYNP6 GSA_SYNP6]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gs/4gsa_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4gsa ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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4GSA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]] with PLP as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.3.8 5.4.3.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=4GSA OCA]].
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*[[Aminomutase 3D structures|Aminomutase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of glutamate-1-semialdehyde aminomutase: an alpha2-dimeric vitamin B6-dependent enzyme with asymmetry in structure and active site reactivity., Hennig M, Grimm B, Contestabile R, John RA, Jansonius JN, Proc Natl Acad Sci U S A. 1997 May 13;94(10):4866-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9144156 9144156]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Synechococcus sp]]
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[[Category: Synechococcus sp.]]
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[[Category: Hennig M]]
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[[Category: Hennig, M.]]
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[[Category: Jansonius JN]]
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[[Category: Jansonius, J.N.]]
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[[Category: PLP]]
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[[Category: asymmetric dimer]]
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[[Category: chlorophyll biosynthesis]]
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[[Category: pyridoxal-5'-phosphate]]
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[[Category: pyridoxamine-5'-phosphate]]
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[[Category: reduced form]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:51:21 2007''
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Current revision

CRYSTAL STRUCTURE OF GLUTAMATE-1-SEMIALDEHYDE AMINOMUTASE (AMINOTRANSFERASE) REDUCED WITH CYANOBOROHYDRATE

PDB ID 4gsa

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