1fyf

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(New page: 200px<br /><applet load="1fyf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fyf, resolution 1.65&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1fyf.gif|left|200px]]<br /><applet load="1fyf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1fyf, resolution 1.65&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A TRUNCATED FORM OF THREONYL-TRNA SYNTHETASE COMPLEXED WITH A SERYL ADENYLATE ANALOG'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A TRUNCATED FORM OF THREONYL-TRNA SYNTHETASE COMPLEXED WITH A SERYL ADENYLATE ANALOG==
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Threonyl-tRNA synthetase, a class II synthetase, uses a unique zinc ion to, discriminate against the isosteric valine at the activation step. The, crystal structure of the enzyme with an analog of seryl adenylate shows, that the noncognate serine cannot be fully discriminated at that step. We, show that hydrolysis of the incorrectly formed ser-tRNA(Thr) is performed, at a specific site in the N-terminal domain of the enzyme. The present, study suggests that both classes of synthetases use effectively the, ability of the CCA end of tRNA to switch between a hairpin and a helical, conformation for aminoacylation and editing. As a consequence, the editing, mechanism of both classes of synthetases can be described as mirror, images, as already seen for tRNA binding and amino acid activation.
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<StructureSection load='1fyf' size='340' side='right'caption='[[1fyf]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fyf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FYF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FYF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SSA:5-O-(N-(L-SERYL)-SULFAMOYL)ADENOSINE'>SSA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fyf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fyf OCA], [https://pdbe.org/1fyf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fyf RCSB], [https://www.ebi.ac.uk/pdbsum/1fyf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fyf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYT_ECOLI SYT_ECOLI] ThrS is also a translational repressor protein, it controls the translation of its own gene by binding to its mRNA.[HAMAP-Rule:MF_00184]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fy/1fyf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fyf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1FYF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and SSA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Threonine--tRNA_ligase Threonine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.3 6.1.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1FYF OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Transfer RNA-mediated editing in threonyl-tRNA synthetase. The class II solution to the double discrimination problem., Dock-Bregeon A, Sankaranarayanan R, Romby P, Caillet J, Springer M, Rees B, Francklyn CS, Ehresmann C, Moras D, Cell. 2000 Dec 8;103(6):877-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11136973 11136973]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Threonine--tRNA ligase]]
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[[Category: Dock-Bregeon AC]]
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[[Category: Dock-Bregeon, A.C.]]
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[[Category: Moras D]]
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[[Category: Moras, D.]]
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[[Category: Sankaranarayanan R]]
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[[Category: Sankaranarayanan, R.]]
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[[Category: SSA]]
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[[Category: ZN]]
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[[Category: adenylate analog]]
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[[Category: amino acid recognition]]
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[[Category: deletion mutant]]
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[[Category: trna-synthetase]]
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[[Category: zinc ion]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:28:11 2007''
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Current revision

CRYSTAL STRUCTURE OF A TRUNCATED FORM OF THREONYL-TRNA SYNTHETASE COMPLEXED WITH A SERYL ADENYLATE ANALOG

PDB ID 1fyf

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