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1g0v

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(New page: 200px<br /><applet load="1g0v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g0v, resolution 2.00&Aring;" /> '''THE STRUCTURE OF PRO...)
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[[Image:1g0v.jpg|left|200px]]<br /><applet load="1g0v" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g0v, resolution 2.00&Aring;" />
 
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'''THE STRUCTURE OF PROTEINASE A COMPLEXED WITH A IA3 MUTANT, MVV'''<br />
 
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==Overview==
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==THE STRUCTURE OF PROTEINASE A COMPLEXED WITH A IA3 MUTANT, MVV==
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The yeast IA3 polypeptide consists of only 68 residues, and the free, inhibitor has little intrinsic secondary structure. IA3 showed, subnanomolar potency toward its target, proteinase A from Saccharomyces, cerevisiae, and did not inhibit any of a large number of aspartic, proteinases with similar sequences/structures from a wide variety of other, species. Systematic truncation and mutagenesis of the IA3 polypeptide, revealed that the inhibitory activity is located in the N-terminal half of, the sequence. Crystal structures of different forms of IA3 complexed with, proteinase A showed that residues in the N-terminal half of the IA3, sequence became ordered and formed an almost perfect alpha-helix in the, active site of the enzyme. This potent, specific interaction was directed, primarily by hydrophobic interactions made by three key features in the, inhibitory sequence. Whereas IA3 was cut as a substrate by the nontarget, aspartic proteinases, it was not cleaved by proteinase A. The random coil, IA3 polypeptide escapes cleavage by being stabilized in a helical, conformation upon interaction with the active site of proteinase A. This, results, paradoxically, in potent selective inhibition of the target, enzyme.
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<StructureSection load='1g0v' size='340' side='right'caption='[[1g0v]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g0v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G0V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g0v OCA], [https://pdbe.org/1g0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g0v RCSB], [https://www.ebi.ac.uk/pdbsum/1g0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g0v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CARP_YEAST CARP_YEAST] Aspartyl protease implicated in the post-translational regulation of S.cerevisiae vacuolar proteinases. Acts on YSCB, on YSCY and on itself.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g0/1g0v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g0v ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1G0V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with MAN and NAG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Saccharopepsin Saccharopepsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.25 3.4.23.25] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G0V OCA].
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*[[Pepsin|Pepsin]]
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*[[Proteinase 3D structures|Proteinase 3D structures]]
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==Reference==
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__TOC__
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The potency and specificity of the interaction between the IA3 inhibitor and its target aspartic proteinase from Saccharomyces cerevisiae., Phylip LH, Lees WE, Brownsey BG, Bur D, Dunn BM, Winther JR, Gustchina A, Li M, Copeland T, Wlodawer A, Kay J, J Biol Chem. 2001 Jan 19;276(3):2023-30. Epub 2000 Oct 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11042188 11042188]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Saccharopepsin]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Brownsey, B.G.]]
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[[Category: Brownsey BG]]
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[[Category: Bur, D.]]
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[[Category: Bur D]]
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[[Category: Copeland, T.]]
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[[Category: Copeland T]]
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[[Category: Dunn, B.M.]]
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[[Category: Dunn BM]]
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[[Category: Gustchina, A.]]
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[[Category: Gustchina A]]
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[[Category: Kay, J.]]
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[[Category: Kay J]]
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[[Category: Lees, W.]]
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[[Category: Lees W]]
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[[Category: Li, M.]]
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[[Category: Li M]]
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[[Category: Phylip, L.H.]]
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[[Category: Phylip LH]]
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[[Category: Winther, J.]]
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[[Category: Winther J]]
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[[Category: Wlodawer, A.]]
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[[Category: Wlodawer A]]
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[[Category: MAN]]
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[[Category: NAG]]
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[[Category: mvv]]
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[[Category: proteinase a]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:36:21 2007''
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Current revision

THE STRUCTURE OF PROTEINASE A COMPLEXED WITH A IA3 MUTANT, MVV

PDB ID 1g0v

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