1h6s

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{{Seed}}
 
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[[Image:1h6s.png|left|200px]]
 
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==Asymmetric conductivity of engineered proteins==
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The line below this paragraph, containing "STRUCTURE_1h6s", creates the "Structure Box" on the page.
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<StructureSection load='1h6s' size='340' side='right'caption='[[1h6s]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1h6s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuscovulum_blasticum Fuscovulum blasticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H6S FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h6s OCA], [https://pdbe.org/1h6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h6s RCSB], [https://www.ebi.ac.uk/pdbsum/1h6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h6s ProSAT]</span></td></tr>
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{{STRUCTURE_1h6s| PDB=1h6s | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PORI_FUSBL PORI_FUSBL] Forms channels that allow the passive diffusion of small hydrophilic solutes up to an exclusion limit of about 0.6 kDa.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h6/1h6s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h6s ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Positively charged peptide segments of 16 and 18 residues were inserted at a periplasmic turn of the porin from Rhodobacter blasticus in order to form an electric field-dependent plug. The X-ray diffraction analysis of a mutant confirmed that the structure of the porin had remained intact and that the insert was mobile. Incorporation experiments of single molecules into lipid bilayers showed that the distribution of electric conduction increments depended on the field polarity. The observed distributions are explained if the porin molecules enter the bilayer preferentially with their periplasmic surface first. Furthermore, the conduction of membrane-incorporated porin mutants changed reproducibly on field reversal showing asymmetries of reverse similar 15%, while the wild-type remained constant. This asymmetry is most likely caused by the electric field pressing the charged insert onto the pore eyelet in one field direction and removing it from the eyelet in the other. The results encourage attempts to improve the inserts in order to eventually reach diode characteristics.
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===ASYMMETRIC CONDUCTIVITY OF ENGINEERED PROTEINS===
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Asymmetric conductivity of engineered porins.,Bannwarth M, Schulz GE Protein Eng. 2002 Oct;15(10):799-804. PMID:12468713<ref>PMID:12468713</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1h6s" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12468713}}, adds the Publication Abstract to the page
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*[[Porin 3D structures|Porin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12468713 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12468713}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Fuscovulum blasticum]]
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1H6S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_blasticus Rhodobacter blasticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H6S OCA].
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[[Category: Large Structures]]
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[[Category: Bannwarth M]]
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==Reference==
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[[Category: Schulz GE]]
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Asymmetric conductivity of engineered porins., Bannwarth M, Schulz GE, Protein Eng. 2002 Oct;15(10):799-804. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12468713 12468713]
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[[Category: Rhodobacter blasticus]]
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[[Category: Single protein]]
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[[Category: Bannwarth, M.]]
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[[Category: Schulz, G E.]]
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[[Category: Integral membrane protein porin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:43:05 2008''
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Asymmetric conductivity of engineered proteins

PDB ID 1h6s

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