1g57

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(New page: 200px<br /><applet load="1g57" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g57, resolution 1.4&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1g57.jpg|left|200px]]<br /><applet load="1g57" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g57, resolution 1.4&Aring;" />
 
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'''CRYSTAL STRUCTURE OF 3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF 3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE==
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BACKGROUND: 3,4-Dihydroxy-2-butanone-4-phosphate synthase catalyzes a, commitment step in the biosynthesis of riboflavin. On the enzyme, ribulose, 5-phosphate is converted to 3,4-dihydroxy-2-butanone 4-phosphate and, formate in steps involving enolization, ketonization, dehydration, skeleton rearrangement, and formate elimination. The enzyme is absent in, humans and an attractive target for the discovery of antimicrobials for, pathogens incapable of acquiring sufficient riboflavin from their hosts., The homodimer of 23 kDa subunits requires Mg(2+) for activity. RESULTS:, The first three-dimensional structure of the enzyme was determined at 1.4, A resolution using the multiwavelength anomalous diffraction (MAD) method, on Escherichia coli protein crystals containing gold. The protein consists, of an alpha + beta fold having a complex linkage of beta strands., Intersubunit contacts are mediated by numerous hydrophobic interactions, and three hydrogen bond networks. CONCLUSIONS: A proposed active site was, identified on the basis of amino acid residues that are conserved among, the enzyme from 19 species. There are two well-separated active sites per, dimer, each of which comprise residues from both subunits. In addition to, three arginines and two threonines, which may be used for recognizing the, phosphate group of the substrate, the active site consists of three, glutamates, two aspartates, two histidines, and a cysteine which may, provide the means for general acid and base catalysis and for coordinating, the Mg(2+) cofactor within the active site.
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<StructureSection load='1g57' size='340' side='right'caption='[[1g57]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g57]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G57 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g57 OCA], [https://pdbe.org/1g57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g57 RCSB], [https://www.ebi.ac.uk/pdbsum/1g57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g57 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIBB_ECOLI RIBB_ECOLI] Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.[HAMAP-Rule:MF_00180]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g57_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g57 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1G57 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G57 OCA].
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*[[Horseradish peroxidase|Horseradish peroxidase]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase of riboflavin biosynthesis., Liao DI, Calabrese JC, Wawrzak Z, Viitanen PV, Jordan DB, Structure. 2001 Jan 10;9(1):11-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11342130 11342130]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Calabrese, J.C.]]
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[[Category: Calabrese JC]]
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[[Category: Jordan, D.B.]]
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[[Category: Jordan DB]]
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[[Category: Liao, D.I.]]
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[[Category: Liao D-I]]
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[[Category: Viitanen, P.V.]]
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[[Category: Viitanen PV]]
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[[Category: Wawrzak, Z.]]
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[[Category: Wawrzak Z]]
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[[Category: CS]]
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[[Category: antimicrobial target]]
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[[Category: dihydroxybutanone phosphate synthase]]
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[[Category: riboflavine biosynthesis]]
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[[Category: skeletal rearrangement]]
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[[Category: structure-based design]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:42:55 2007''
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Current revision

CRYSTAL STRUCTURE OF 3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE

PDB ID 1g57

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