1g59

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(New page: 200px<br /><applet load="1g59" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g59, resolution 2.4&Aring;" /> '''GLUTAMYL-TRNA SYNTHET...)
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[[Image:1g59.gif|left|200px]]<br /><applet load="1g59" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g59, resolution 2.4&Aring;" />
 
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'''GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).'''<br />
 
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==Overview==
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==GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).==
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Glutamyl-tRNA synthetases (GluRSs) are divided into two distinct types, with regard to the presence or absence of glutaminyl-tRNA synthetase, (GlnRS) in the genetic translation systems. In the original 19-synthetase, systems lacking GlnRS, the 'non-discriminating' GluRS glutamylates both, tRNAGlu and tRNAGln. In contrast, in the evolved 20-synthetase systems, with GlnRS, the 'discriminating' GluRS aminoacylates only tRNAGlu. Here we, report the 2.4 A resolution crystal structure of a 'discriminating', GluRS.tRNAGlu complex from Thermus thermophilus. The GluRS recognizes the, tRNAGlu anticodon bases via two alpha-helical domains, maintaining the, base stacking. We show that the discrimination between the Glu and Gln, anticodons (34YUC36 and 34YUG36, respectively) is achieved by a single, arginine residue (Arg 358). The mutation of Arg 358 to Gln resulted in a, GluRS that does not discriminate between the Glu and Gln anticodons. This, change mimics the reverse course of GluRS evolution from anticodon, 'non-dicsriminating' to 'discriminating'.
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<StructureSection load='1g59' size='340' side='right'caption='[[1g59]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g59]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G59 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g59 OCA], [https://pdbe.org/1g59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g59 RCSB], [https://www.ebi.ac.uk/pdbsum/1g59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g59 ProSAT], [https://www.topsan.org/Proteins/RSGI/1g59 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYE_THET8 SYE_THET8] Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).<ref>PMID:11224561</ref> <ref>PMID:17161369</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g59_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g59 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glutamyl-tRNA synthetases (GluRSs) are divided into two distinct types, with regard to the presence or absence of glutaminyl-tRNA synthetase (GlnRS) in the genetic translation systems. In the original 19-synthetase systems lacking GlnRS, the 'non-discriminating' GluRS glutamylates both tRNAGlu and tRNAGln. In contrast, in the evolved 20-synthetase systems with GlnRS, the 'discriminating' GluRS aminoacylates only tRNAGlu. Here we report the 2.4 A resolution crystal structure of a 'discriminating' GluRS.tRNAGlu complex from Thermus thermophilus. The GluRS recognizes the tRNAGlu anticodon bases via two alpha-helical domains, maintaining the base stacking. We show that the discrimination between the Glu and Gln anticodons (34YUC36 and 34YUG36, respectively) is achieved by a single arginine residue (Arg 358). The mutation of Arg 358 to Gln resulted in a GluRS that does not discriminate between the Glu and Gln anticodons. This change mimics the reverse course of GluRS evolution from anticodon 'non-dicsriminating' to 'discriminating'.
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==About this Structure==
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Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase.,Sekine S, Nureki O, Shimada A, Vassylyev DG, Yokoyama S Nat Struct Biol. 2001 Mar;8(3):203-6. PMID:11224561<ref>PMID:11224561</ref>
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1G59 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Active as [http://en.wikipedia.org/wiki/Glutamate--tRNA_ligase Glutamate--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.17 6.1.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G59 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase., Sekine S, Nureki O, Shimada A, Vassylyev DG, Yokoyama S, Nat Struct Biol. 2001 Mar;8(3):203-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11224561 11224561]
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</div>
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[[Category: Glutamate--tRNA ligase]]
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<div class="pdbe-citations 1g59" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
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[[Category: Nureki, O.]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Sekine, S.]]
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[[Category: Shimada, A.]]
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[[Category: Vassylyev, D.G.]]
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[[Category: Yokoyama, S.]]
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[[Category: aminoacyl-trna synthetase]]
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[[Category: protein-rna complex]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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[[Category: transfer rna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:43:04 2007''
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==See Also==
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus]]
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[[Category: Nureki O]]
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[[Category: Sekine S]]
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[[Category: Shimada A]]
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[[Category: Vassylyev DG]]
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[[Category: Yokoyama S]]

Current revision

GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).

PDB ID 1g59

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