1g5i

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1g5i" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g5i, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
Current revision (06:08, 9 August 2023) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1g5i.gif|left|200px]]<br /><applet load="1g5i" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1g5i, resolution 2.3&Aring;" />
 
-
'''CRYSTAL STRUCTURE OF THE ACCESSORY SUBUNIT OF MURINE MITOCHONDRIAL POLYMERASE GAMMA'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURE OF THE ACCESSORY SUBUNIT OF MURINE MITOCHONDRIAL POLYMERASE GAMMA==
-
Polymerase gamma, which replicates and repairs mitochondrial DNA, requires, the Pol gamma B subunit for processivity. We determined the crystal, structure of mouse Pol gamma B, a core component of the mitochondrial, replication machinery. Pol gamma B shows high similarity to glycyl-tRNA, synthetase and dimerizes through an unusual intermolecular four-helix, bundle. A human Pol gamma B mutant lacking the four-helix bundle failed to, dimerize in solution or to stimulate the catalytic subunit Pol gamma A, but retained the ability to bind with Pol gamma A to a primer-template, construct, indicating that the functional holoenzyme contains two Pol, gamma B molecules. Other mutants retained stimulatory activity but lost, the ability to bind folded ssDNA. These results suggest that the Pol gamma, B dimer contains distinct sites for Pol gamma A binding, dimerization, and, DNA binding.
+
<StructureSection load='1g5i' size='340' side='right'caption='[[1g5i]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1g5i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5I FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5i OCA], [https://pdbe.org/1g5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5i RCSB], [https://www.ebi.ac.uk/pdbsum/1g5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5i ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DPOG2_MOUSE DPOG2_MOUSE] Mitochondrial polymerase processivity subunit. Stimulates the polymerase and exonuclease activities, and increases the processivity of the enzyme. Binds to ss-DNA.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5i_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5i ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Polymerase gamma, which replicates and repairs mitochondrial DNA, requires the Pol gamma B subunit for processivity. We determined the crystal structure of mouse Pol gamma B, a core component of the mitochondrial replication machinery. Pol gamma B shows high similarity to glycyl-tRNA synthetase and dimerizes through an unusual intermolecular four-helix bundle. A human Pol gamma B mutant lacking the four-helix bundle failed to dimerize in solution or to stimulate the catalytic subunit Pol gamma A, but retained the ability to bind with Pol gamma A to a primer-template construct, indicating that the functional holoenzyme contains two Pol gamma B molecules. Other mutants retained stimulatory activity but lost the ability to bind folded ssDNA. These results suggest that the Pol gamma B dimer contains distinct sites for Pol gamma A binding, dimerization, and DNA binding.
-
==About this Structure==
+
Crystal structure and deletion analysis show that the accessory subunit of mammalian DNA polymerase gamma, Pol gamma B, functions as a homodimer.,Carrodeguas JA, Theis K, Bogenhagen DF, Kisker C Mol Cell. 2001 Jan;7(1):43-54. PMID:11172710<ref>PMID:11172710</ref>
-
1G5I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NA and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G5I OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Crystal structure and deletion analysis show that the accessory subunit of mammalian DNA polymerase gamma, Pol gamma B, functions as a homodimer., Carrodeguas JA, Theis K, Bogenhagen DF, Kisker C, Mol Cell. 2001 Jan;7(1):43-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11172710 11172710]
+
</div>
-
[[Category: Mus musculus]]
+
<div class="pdbe-citations 1g5i" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Bogenhagen, D.F.]]
+
-
[[Category: Carrodeguas, J.A.]]
+
-
[[Category: Kisker, C.]]
+
-
[[Category: Theis, K.]]
+
-
[[Category: GOL]]
+
-
[[Category: NA]]
+
-
[[Category: intermolecular four helix bundle]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:43:28 2007''
+
==See Also==
 +
*[[Horseradish peroxidase|Horseradish peroxidase]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Mus musculus]]
 +
[[Category: Bogenhagen DF]]
 +
[[Category: Carrodeguas JA]]
 +
[[Category: Kisker C]]
 +
[[Category: Theis K]]

Current revision

CRYSTAL STRUCTURE OF THE ACCESSORY SUBUNIT OF MURINE MITOCHONDRIAL POLYMERASE GAMMA

PDB ID 1g5i

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g5i"
Personal tools