1g5p

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(New page: 200px<br /><applet load="1g5p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g5p, resolution 2.2&Aring;" /> '''NITROGENASE IRON PROT...)
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[[Image:1g5p.jpg|left|200px]]<br /><applet load="1g5p" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g5p, resolution 2.2&Aring;" />
 
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'''NITROGENASE IRON PROTEIN FROM AZOTOBACTER VINELANDII'''<br />
 
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==Overview==
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==NITROGENASE IRON PROTEIN FROM AZOTOBACTER VINELANDII==
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The structure of the nitrogenase iron protein from Azotobacter vinelandii, in the all-ferrous [4Fe-4S](0) form has been determined to 2.25 A, resolution by using the multiwavelength anomalous diffraction (MAD), phasing technique. The structure demonstrates that major conformational, changes are not necessary either in the iron protein or in the cluster to, accommodate cluster reduction to the [4Fe-4S](0) oxidation state. A survey, of [4Fe-4S] clusters coordinated by four cysteine ligands in proteins of, known structure reveals that the [4Fe-4S] cluster of the iron protein has, the largest accessible surface area, suggesting that solvent exposure may, be relevant to the ability of the iron protein to exist in three oxidation, states.
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<StructureSection load='1g5p' size='340' side='right'caption='[[1g5p]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g5p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G5P FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g5p OCA], [https://pdbe.org/1g5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g5p RCSB], [https://www.ebi.ac.uk/pdbsum/1g5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g5p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NIFH1_AZOVI NIFH1_AZOVI] The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein (component 2) and a component 1 which is either a molybdenum-iron protein, a vanadium-iron, or an iron-iron protein.[HAMAP-Rule:MF_00533]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g5/1g5p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g5p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the nitrogenase iron protein from Azotobacter vinelandii in the all-ferrous [4Fe-4S](0) form has been determined to 2.25 A resolution by using the multiwavelength anomalous diffraction (MAD) phasing technique. The structure demonstrates that major conformational changes are not necessary either in the iron protein or in the cluster to accommodate cluster reduction to the [4Fe-4S](0) oxidation state. A survey of [4Fe-4S] clusters coordinated by four cysteine ligands in proteins of known structure reveals that the [4Fe-4S] cluster of the iron protein has the largest accessible surface area, suggesting that solvent exposure may be relevant to the ability of the iron protein to exist in three oxidation states.
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==About this Structure==
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Crystal structure of the all-ferrous [4Fe-4S]0 form of the nitrogenase iron protein from Azotobacter vinelandii.,Strop P, Takahara PM, Chiu H, Angove HC, Burgess BK, Rees DC Biochemistry. 2001 Jan 23;40(3):651-6. PMID:11170381<ref>PMID:11170381</ref>
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1G5P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with SF4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nitrogenase Nitrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.6.1 1.18.6.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G5P OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the all-ferrous [4Fe-4S]0 form of the nitrogenase iron protein from Azotobacter vinelandii., Strop P, Takahara PM, Chiu H, Angove HC, Burgess BK, Rees DC, Biochemistry. 2001 Jan 23;40(3):651-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11170381 11170381]
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</div>
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[[Category: Azotobacter vinelandii]]
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<div class="pdbe-citations 1g5p" style="background-color:#fffaf0;"></div>
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[[Category: Nitrogenase]]
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[[Category: Single protein]]
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[[Category: Angove, H.C.]]
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[[Category: Burgess, B.K.]]
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[[Category: Chiu, H.J.]]
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[[Category: Rees, D.C.]]
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[[Category: Strop, P.]]
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[[Category: Takahara, P.M.]]
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[[Category: SF4]]
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[[Category: iron protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:43:40 2007''
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==See Also==
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*[[Nitrogenase 3D structures|Nitrogenase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Azotobacter vinelandii]]
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[[Category: Large Structures]]
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[[Category: Angove HC]]
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[[Category: Burgess BK]]
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[[Category: Chiu HJ]]
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[[Category: Rees DC]]
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[[Category: Strop P]]
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[[Category: Takahara PM]]

Current revision

NITROGENASE IRON PROTEIN FROM AZOTOBACTER VINELANDII

PDB ID 1g5p

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