1g6t

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(New page: 200px<br /><applet load="1g6t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g6t, resolution 1.60&Aring;" /> '''STRUCTURE OF EPSP SY...)
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[[Image:1g6t.jpg|left|200px]]<br /><applet load="1g6t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g6t, resolution 1.60&Aring;" />
 
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'''STRUCTURE OF EPSP SYNTHASE LIGANDED WITH SHIKIMATE-3-PHOSPHATE'''<br />
 
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==Overview==
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==STRUCTURE OF EPSP SYNTHASE LIGANDED WITH SHIKIMATE-3-PHOSPHATE==
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Biosynthesis of aromatic amino acids in plants, many bacteria, and, microbes relies on the enzyme 5-enolpyruvylshikimate 3-phosphate (EPSP), synthase, a prime target for drugs and herbicides. We have identified the, interaction of EPSP synthase with one of its two substrates (shikimate, 3-phosphate) and with the widely used herbicide glyphosate by x-ray, crystallography. The two-domain enzyme closes on ligand binding, thereby, forming the active site in the interdomain cleft. Glyphosate appears to, occupy the binding site of the second substrate of EPSP synthase, (phosphoenol pyruvate), mimicking an intermediate state of the ternary, enzyme.substrates complex. The elucidation of the active site of EPSP, synthase and especially of the binding pattern of glyphosate provides a, valuable roadmap for engineering new herbicides and herbicide-resistant, crops, as well as new antibiotic and antiparasitic drugs.
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<StructureSection load='1g6t' size='340' side='right'caption='[[1g6t]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g6t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G6T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=S3P:SHIKIMATE-3-PHOSPHATE'>S3P</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g6t OCA], [https://pdbe.org/1g6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g6t RCSB], [https://www.ebi.ac.uk/pdbsum/1g6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g6t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AROA_ECOLI AROA_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g6/1g6t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g6t ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1G6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4, S3P and FMT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G6T OCA].
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*[[EPSP synthase 3D structures|EPSP synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail., Schonbrunn E, Eschenburg S, Shuttleworth WA, Schloss JV, Amrhein N, Evans JN, Kabsch W, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1376-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11171958 11171958]
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[[Category: 3-phosphoshikimate 1-carboxyvinyltransferase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Amrhein, N.]]
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[[Category: Amrhein N]]
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[[Category: Eschenburg, S.]]
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[[Category: Eschenburg S]]
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[[Category: Evans, J.N.S.]]
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[[Category: Evans JNS]]
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[[Category: Kabsch, W.]]
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[[Category: Kabsch W]]
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[[Category: Schloss, J.V.]]
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[[Category: Schloss JV]]
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[[Category: Schonbrunn, E.]]
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[[Category: Schonbrunn E]]
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[[Category: Shuttleworth, W.]]
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[[Category: Shuttleworth W]]
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[[Category: FMT]]
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[[Category: PO4]]
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[[Category: S3P]]
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[[Category: two-domain structure; inside-out alpha-beta barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:45:53 2007''
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Current revision

STRUCTURE OF EPSP SYNTHASE LIGANDED WITH SHIKIMATE-3-PHOSPHATE

PDB ID 1g6t

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