1g7r

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(New page: 200px<br /><applet load="1g7r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g7r, resolution 2.2&Aring;" /> '''X-RAY STRUCTURE OF TR...)
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[[Image:1g7r.jpg|left|200px]]<br /><applet load="1g7r" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g7r, resolution 2.2&Aring;" />
 
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'''X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B'''<br />
 
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==Overview==
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==X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B==
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X-ray structures of the universal translation initiation factor IF2/eIF5B, have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that, facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes, in all three kingdoms of life. The conserved core of IF2/eIF5B consists of, an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed, by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to, a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a, molecular lever, which amplifies a modest conformational change in the, Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a, distance of 90 A from the G domain active center to domain IV. Mechanisms, of GTPase function and ribosome binding are discussed.
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<StructureSection load='1g7r' size='340' side='right'caption='[[1g7r]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G7R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g7r OCA], [https://pdbe.org/1g7r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g7r RCSB], [https://www.ebi.ac.uk/pdbsum/1g7r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g7r ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/1g7r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g7r ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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X-ray structures of the universal translation initiation factor IF2/eIF5B have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes in all three kingdoms of life. The conserved core of IF2/eIF5B consists of an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a molecular lever, which amplifies a modest conformational change in the Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a distance of 90 A from the G domain active center to domain IV. Mechanisms of GTPase function and ribosome binding are discussed.
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==About this Structure==
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X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding.,Roll-Mecak A, Cao C, Dever TE, Burley SK Cell. 2000 Nov 22;103(5):781-92. PMID:11114334<ref>PMID:11114334</ref>
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1G7R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G7R OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding., Roll-Mecak A, Cao C, Dever TE, Burley SK, Cell. 2000 Nov 22;103(5):781-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11114334 11114334]
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</div>
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[[Category: Methanothermobacter thermautotrophicus]]
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<div class="pdbe-citations 1g7r" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Burley, S.K.]]
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<references/>
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[[Category: Cao, C.]]
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__TOC__
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[[Category: Dever, T.E.]]
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</StructureSection>
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[[Category: Roll-Mecak, A.]]
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[[Category: Large Structures]]
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[[Category: translational gtpase]]
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[[Category: Burley SK]]
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[[Category: Cao C]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:47:30 2007''
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[[Category: Dever TE]]
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[[Category: Roll-Mecak A]]

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X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B

PDB ID 1g7r

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