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1g8k

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CRYSTAL STRUCTURE ANALYSIS OF ARSENITE OXIDASE FROM ALCALIGENES FAECALIS

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-[[Image:1g8k.gif|left|200px]]<br /><applet load="1g8k" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1g8k, resolution 1.64&Aring;" />
-'''CRYSTAL STRUCTURE ANALYSIS OF ARSENITE OXIDASE FROM ALCALIGENES FAECALIS'''<br />
-==Overview==
+==CRYSTAL STRUCTURE ANALYSIS OF ARSENITE OXIDASE FROM ALCALIGENES FAECALIS==
-BACKGROUND: Arsenite oxidase from Alcaligenes faecalis NCIB 8687 is a, molybdenum/iron protein involved in the detoxification of arsenic. It is, induced by the presence of AsO(2-) (arsenite) and functions to oxidize, As(III)O(2-), which binds to essential sulfhydryl groups of proteins and, dithiols, to the relatively less toxic As(V)O(4)(3-) (arsenate) prior to, methylation. RESULTS: Using a combination of multiple isomorphous, replacement with anomalous scattering (MIRAS) and multiple-wavelength, anomalous dispersion (MAD) methods, the crystal structure of arsenite, oxidase was determined to 2.03 A in a P2(1) crystal form with two, molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with, four molecules in the asymmetric unit. Arsenite oxidase consists of a, large subunit of 825 residues and a small subunit of approximately 134, residues. The large subunit contains a Mo site, consisting of a Mo atom, bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit, contains a Rieske-type [2Fe-2S] site. CONCLUSIONS: The large subunit of, arsenite oxidase is similar to other members of the dimethylsulfoxide, (DMSO) reductase family of molybdenum enzymes, particularly the, dissimilatory periplasmic nitrate reductase from Desulfovibrio, desulfuricans, but is unique in having no covalent bond between the, polypeptide and the Mo atom. The small subunit has no counterpart among, known Mo protein structures but is homologous to the Rieske [2Fe-2S], protein domain of the cytochrome bc(1) and cytochrome b(6)f complexes and, to the Rieske domain of naphthalene 1,2-dioxygenase.
+<StructureSection load='1g8k' size='340' side='right'caption='[[1g8k]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1g8k]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G8K FirstGlance]. <br>
-G8K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with HG, CA, MGD, O, 4MO, F3S, FES and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G8K OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4MO:MOLYBDENUM(IV)+ION'>4MO</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=O:OXYGEN+ATOM'>O</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g8k OCA], [https://pdbe.org/1g8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g8k RCSB], [https://www.ebi.ac.uk/pdbsum/1g8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g8k ProSAT]</span></td></tr>
-Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A., Ellis PJ, Conrads T, Hille R, Kuhn P, Structure. 2001 Feb 7;9(2):125-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11250197 11250197]
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AIOA_ALCFA AIOA_ALCFA] Involved in the detoxification of arsenic. Oxidizes As(III)O3(3-) (arsenite) to the somewhat less toxic As(V)O4(3-) (arsenate).
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g8/1g8k_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g8k ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
 [[Category: Alcaligenes faecalis]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Conrads, T.]]
+[[Category: Conrads T]]
-[[Category: Ellis, P.J.]]
+[[Category: Ellis PJ]]
-[[Category: Hille, R.]]
+[[Category: Hille R]]
-[[Category: Kuhn, P.]]
+[[Category: Kuhn P]]
-[[Category: 4MO]]
-[[Category: CA]]
-[[Category: EDO]]
-[[Category: F3S]]
-[[Category: FES]]
-[[Category: HG]]
-[[Category: MGD]]
-[[Category: O]]
-[[Category: [2fe-2s] cluster]]
-[[Category: [3fe-4s] cluster]]
-[[Category: arsenite]]
-[[Category: molybdopterin]]
-[[Category: oxidase]]
-[[Category: rieske]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:49:03 2007''

1g8k

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CRYSTAL STRUCTURE ANALYSIS OF ARSENITE OXIDASE FROM ALCALIGENES FAECALIS

Structural highlights

Function

Evolutionary Conservation

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