1g8o

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(New page: 200px<br /><applet load="1g8o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g8o, resolution 2.30&Aring;" /> '''CRYSTALLOGRAPHIC STR...)
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[[Image:1g8o.jpg|left|200px]]<br /><applet load="1g8o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g8o, resolution 2.30&Aring;" />
 
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'''CRYSTALLOGRAPHIC STRUCTURE OF THE NATIVE BOVINE ALPHA-1,3-GALACTOSYLTRANSFERASE CATALYTIC DOMAIN'''<br />
 
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==Overview==
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==CRYSTALLOGRAPHIC STRUCTURE OF THE NATIVE BOVINE ALPHA-1,3-GALACTOSYLTRANSFERASE CATALYTIC DOMAIN==
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alpha1,3-galactosyltransferase (alpha3GalT, EC 2.4.1.151) is a, Golgi-resident, type II transmembrane protein that transfers galactose, from UDP-alpha-galactose to the terminal N:-acetyllactosamine unit of, glycoconjugate glycans, producing the Galalpha1,3Galbeta1,4GlcNAc, oligosaccharide structure present in most mammalian glycoproteins. Unlike, most other mammals, humans and Old World primates do not possess, alpha3GalT activity, which is relevant for the hyperacute rejection, observed in pig-to-human xenotransplantation. The crystal structure of the, catalytic domain of substrate-free bovine alpha3GalT, solved and refined, to 2.3 A resolution, has a globular shape with an alpha/beta fold, containing a narrow cleft on one face, and shares a UDP-binding domain, (UBD) with the recently solved inverting glycosyltransferases. The, substrate-bound complex, solved and refined to 2.5 A, allows the, description of residues interacting directly with UDP-galactose. These, structural data suggest that the strictly conserved residue E317 is likely, to be the catalytic nucleophile involved in galactose transfer with, retention of anomeric configuration as accomplished by this enzyme., Moreover, the alpha3GalT structure helps to identify amino acid residues, that determine the specificities of the highly homologous ABO histo-blood, group and glycosphingolipid glycosyltransferases.
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<StructureSection load='1g8o' size='340' side='right'caption='[[1g8o]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g8o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G8O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g8o OCA], [https://pdbe.org/1g8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g8o RCSB], [https://www.ebi.ac.uk/pdbsum/1g8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g8o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g8/1g8o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g8o ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1G8O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MN and U5P as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G8O OCA].
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Bovine alpha1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases., Gastinel LN, Bignon C, Misra AK, Hindsgaul O, Shaper JH, Joziasse DH, EMBO J. 2001 Feb 15;20(4):638-49. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11179209 11179209]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Bigon C]]
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[[Category: Bigon, C.]]
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[[Category: Gastinel LN]]
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[[Category: Gastinel, L.N.]]
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[[Category: Hindsgaul O]]
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[[Category: Hindsgaul, O.]]
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[[Category: Joziasse DH]]
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[[Category: Joziasse, D.H.]]
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[[Category: Misra AK]]
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[[Category: Misra, A.K.]]
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[[Category: Shaper JH]]
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[[Category: Shaper, J.H.]]
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[[Category: MN]]
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[[Category: U5P]]
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[[Category: alpha-beta-alpha]]
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[[Category: udp binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:49:18 2007''
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Current revision

CRYSTALLOGRAPHIC STRUCTURE OF THE NATIVE BOVINE ALPHA-1,3-GALACTOSYLTRANSFERASE CATALYTIC DOMAIN

PDB ID 1g8o

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