1g93

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(New page: 200px<br /><applet load="1g93" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g93, resolution 2.5&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1g93.jpg|left|200px]]<br /><applet load="1g93" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1g93, resolution 2.5&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE BOVINE CATALYTIC DOMAIN OF ALPHA-1,3-GALACTOSYLTRANSFERASE IN THE PRESENCE OF UDP-GALACTOSE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE BOVINE CATALYTIC DOMAIN OF ALPHA-1,3-GALACTOSYLTRANSFERASE IN THE PRESENCE OF UDP-GALACTOSE==
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alpha1,3-galactosyltransferase (alpha3GalT, EC 2.4.1.151) is a, Golgi-resident, type II transmembrane protein that transfers galactose, from UDP-alpha-galactose to the terminal N:-acetyllactosamine unit of, glycoconjugate glycans, producing the Galalpha1,3Galbeta1,4GlcNAc, oligosaccharide structure present in most mammalian glycoproteins. Unlike, most other mammals, humans and Old World primates do not possess, alpha3GalT activity, which is relevant for the hyperacute rejection, observed in pig-to-human xenotransplantation. The crystal structure of the, catalytic domain of substrate-free bovine alpha3GalT, solved and refined, to 2.3 A resolution, has a globular shape with an alpha/beta fold, containing a narrow cleft on one face, and shares a UDP-binding domain, (UBD) with the recently solved inverting glycosyltransferases. The, substrate-bound complex, solved and refined to 2.5 A, allows the, description of residues interacting directly with UDP-galactose. These, structural data suggest that the strictly conserved residue E317 is likely, to be the catalytic nucleophile involved in galactose transfer with, retention of anomeric configuration as accomplished by this enzyme., Moreover, the alpha3GalT structure helps to identify amino acid residues, that determine the specificities of the highly homologous ABO histo-blood, group and glycosphingolipid glycosyltransferases.
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<StructureSection load='1g93' size='340' side='right'caption='[[1g93]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1g93]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G93 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1G93 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UPG:URIDINE-5-DIPHOSPHATE-GLUCOSE'>UPG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1g93 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1g93 OCA], [https://pdbe.org/1g93 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1g93 RCSB], [https://www.ebi.ac.uk/pdbsum/1g93 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1g93 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g9/1g93_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1g93 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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alpha1,3-galactosyltransferase (alpha3GalT, EC 2.4.1.151) is a Golgi-resident, type II transmembrane protein that transfers galactose from UDP-alpha-galactose to the terminal N:-acetyllactosamine unit of glycoconjugate glycans, producing the Galalpha1,3Galbeta1,4GlcNAc oligosaccharide structure present in most mammalian glycoproteins. Unlike most other mammals, humans and Old World primates do not possess alpha3GalT activity, which is relevant for the hyperacute rejection observed in pig-to-human xenotransplantation. The crystal structure of the catalytic domain of substrate-free bovine alpha3GalT, solved and refined to 2.3 A resolution, has a globular shape with an alpha/beta fold containing a narrow cleft on one face, and shares a UDP-binding domain (UBD) with the recently solved inverting glycosyltransferases. The substrate-bound complex, solved and refined to 2.5 A, allows the description of residues interacting directly with UDP-galactose. These structural data suggest that the strictly conserved residue E317 is likely to be the catalytic nucleophile involved in galactose transfer with retention of anomeric configuration as accomplished by this enzyme. Moreover, the alpha3GalT structure helps to identify amino acid residues that determine the specificities of the highly homologous ABO histo-blood group and glycosphingolipid glycosyltransferases.
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==About this Structure==
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Bovine alpha1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases.,Gastinel LN, Bignon C, Misra AK, Hindsgaul O, Shaper JH, Joziasse DH EMBO J. 2001 Feb 15;20(4):638-49. PMID:11179209<ref>PMID:11179209</ref>
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1G93 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with GAL, MN, HG and UPG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1G93 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Bovine alpha1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases., Gastinel LN, Bignon C, Misra AK, Hindsgaul O, Shaper JH, Joziasse DH, EMBO J. 2001 Feb 15;20(4):638-49. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11179209 11179209]
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</div>
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[[Category: Bos taurus]]
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<div class="pdbe-citations 1g93" style="background-color:#fffaf0;"></div>
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[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]
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[[Category: Single protein]]
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[[Category: Bignon, C.]]
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[[Category: Gastinel, L.N.]]
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[[Category: Hindsgaul, O.]]
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[[Category: Joziasse, D.H.]]
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[[Category: Misra, A.K.]]
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[[Category: Shaper, J.H.]]
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[[Category: GAL]]
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[[Category: HG]]
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[[Category: MN]]
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[[Category: UPG]]
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[[Category: alpha-beta-alpha]]
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[[Category: glycosyltransferase]]
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[[Category: udp binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:50:09 2007''
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==See Also==
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*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Large Structures]]
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[[Category: Bignon C]]
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[[Category: Gastinel LN]]
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[[Category: Hindsgaul O]]
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[[Category: Joziasse DH]]
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[[Category: Misra AK]]
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[[Category: Shaper JH]]

Current revision

CRYSTAL STRUCTURE OF THE BOVINE CATALYTIC DOMAIN OF ALPHA-1,3-GALACTOSYLTRANSFERASE IN THE PRESENCE OF UDP-GALACTOSE

PDB ID 1g93

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