1i0x

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{{Seed}}
 
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[[Image:1i0x.png|left|200px]]
 
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==RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL)==
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The line below this paragraph, containing "STRUCTURE_1i0x", creates the "Structure Box" on the page.
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<StructureSection load='1i0x' size='340' side='right'caption='[[1i0x]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1i0x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I0X FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2GP:GUANOSINE-2-MONOPHOSPHATE'>2GP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_1i0x| PDB=1i0x | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i0x OCA], [https://pdbe.org/1i0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i0x RCSB], [https://www.ebi.ac.uk/pdbsum/1i0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i0x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNT1_ASPOR RNT1_ASPOR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i0/1i0x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i0x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the crystalline state, ribonuclease T1 binds calcium ions at different lattice-dependent positions. In solution, its conformational stability is also remarkably increased in the presence of divalent metal ions. Combining urea unfolding studies and X-ray crystallography, we compared the presence of several metal ions at specific sites in the protein to their contribution to the overall stabilizing effect in solution. We constructed thermodynamic cycles involving particular metal ions and specific carboxylate functions. The resulting coupling energies indicate that some (but not all) metal ions found at lattice contacts in crystal structures may indeed significantly contribute to stability enhancement in the presence of metal ions in solution.
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===RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL)===
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The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution.,Deswarte J, De Vos S, Langhorst U, Steyaert J, Loris R Eur J Biochem. 2001 Jul;268(14):3993-4000. PMID:11453993<ref>PMID:11453993</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1i0x" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11453993}}, adds the Publication Abstract to the page
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11453993 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11453993}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1I0X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0X OCA].
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==Reference==
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The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution., Deswarte J, De Vos S, Langhorst U, Steyaert J, Loris R, Eur J Biochem. 2001 Jul;268(14):3993-4000. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11453993 11453993]
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[[Category: Aspergillus oryzae]]
[[Category: Aspergillus oryzae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Langhorst, U.]]
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[[Category: De Swarte J]]
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[[Category: Loris, R.]]
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[[Category: De Vos S]]
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[[Category: Steyaert, J.]]
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[[Category: Langhorst U]]
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[[Category: Swarte, J De.]]
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[[Category: Loris R]]
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[[Category: Vos, S De.]]
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[[Category: Steyaert J]]
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[[Category: 2'gmp]]
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[[Category: Metal binding]]
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[[Category: Ribonuclease]]
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[[Category: Rnase]]
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[[Category: Stability]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:14:46 2008''
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Current revision

RIBONUCLEASE T1 IN COMPLEX WITH 2'GMP (FORM II CRYSTAL)

PDB ID 1i0x

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