1i4v

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{{Seed}}
 
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[[Image:1i4v.png|left|200px]]
 
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==SOLUTION STRUCTURE OF THE UMUD' HOMODIMER==
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The line below this paragraph, containing "STRUCTURE_1i4v", creates the "Structure Box" on the page.
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<StructureSection load='1i4v' size='340' side='right'caption='[[1i4v]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1i4v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I4V FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i4v OCA], [https://pdbe.org/1i4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i4v RCSB], [https://www.ebi.ac.uk/pdbsum/1i4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i4v ProSAT]</span></td></tr>
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{{STRUCTURE_1i4v| PDB=1i4v | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UMUD_ECOLI UMUD_ECOLI] Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/1i4v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i4v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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During the SOS response of Escherichia coli to DNA damage, the umuDC operon is induced, producing the trimeric protein complexes UmuD2C, a DNA damage checkpoint effector, and UmuD'2C (DNA polymerase V), which carries out translesion synthesis, the basis of 'SOS mutagenesis'. UmuD'2, the homodimeric component of DNA pol V, is produced from UmuD by RecA-facilitated self-cleavage, which removes the 24 N-terminal residues of UmuD. We report the solution structure of UmuD'2 (PDB ID 1I4V) and interactions within UmuD'-UmuD, a heterodimer inactive in translesion synthesis. The overall shape of UmuD'2 in solution differs substantially from the previously reported crystal structure, even though the topologies of the two structures are quite similar. Most significantly, the active site residues S60 and K97 do not point directly at one another in solution as they do in the crystal, suggesting that self-cleavage of UmuD might require RecA to assemble the active site. Structural differences between UmuD'2 and UmuD'- UmuD suggest that UmuD'2C and UmuD2C might achieve their different biological activities through distinct interactions with RecA and DNA pol III.
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===SOLUTION STRUCTURE OF THE UMUD' HOMODIMER===
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Converting a DNA damage checkpoint effector (UmuD2C) into a lesion bypass polymerase (UmuD'2C).,Ferentz AE, Walker GC, Wagner G EMBO J. 2001 Aug 1;20(15):4287-98. PMID:11483531<ref>PMID:11483531</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_11483531}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1i4v" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11483531 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11483531}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1I4V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4V OCA].
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==Reference==
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Converting a DNA damage checkpoint effector (UmuD2C) into a lesion bypass polymerase (UmuD'2C)., Ferentz AE, Walker GC, Wagner G, EMBO J. 2001 Aug 1;20(15):4287-98. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11483531 11483531]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ferentz, A E.]]
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[[Category: Ferentz AE]]
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[[Category: Wagner, G.]]
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[[Category: Wagner G]]
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[[Category: Walker, G C.]]
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[[Category: Walker GC]]
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[[Category: Autocatalytic cleavage]]
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[[Category: Dna polymerase accessory protein]]
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[[Category: Dna polymerase v]]
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[[Category: Dna repair]]
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[[Category: Lambda ci]]
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[[Category: Lexa repressor]]
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[[Category: Serine protease]]
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[[Category: Serine-lysine dyad]]
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[[Category: Signal peptidase]]
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[[Category: Sos mutagenesis]]
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[[Category: Sos response]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:25:21 2008''
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Current revision

SOLUTION STRUCTURE OF THE UMUD' HOMODIMER

PDB ID 1i4v

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