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1gct

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(New page: 200px<br /><applet load="1gct" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gct, resolution 1.6&Aring;" /> '''IS GAMMA-CHYMOTRYPSIN...)
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[[Image:1gct.gif|left|200px]]<br /><applet load="1gct" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gct, resolution 1.6&Aring;" />
 
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'''IS GAMMA-CHYMOTRYPSIN A TETRAPEPTIDE ACYL-ENZYME ADDUCT OF GAMMA-CHYMOTRYPSIN?'''<br />
 
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==Overview==
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==IS GAMMA-CHYMOTRYPSIN A TETRAPEPTIDE ACYL-ENZYME ADDUCT OF GAMMA-CHYMOTRYPSIN?==
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Refinement of the structure of gamma-chymotrypsin based on X-ray, crystallographic data to 1.6-A resolution has confirmed the overall, conformation of the molecule as reported previously [Cohen, G. H., Silverton, E. W., &amp; Davies, D. R. (1981) J. Mol. Biol. 148, 449-479]. In, addition, the new refinement suggests that gamma-chymotrypsin, which is, operationally defined by its crystalline habit, may not be the free enzyme, but rather a complex, possibly an acyl-enzyme adduct, with the, tetrapeptide Pro-Gly-Ala-Tyr (or a close homologue). The crystallographic, refinement provides a detailed geometrical description of the, enzyme-substrate-solvent interactions that occur in the presumptive, adduct.
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<StructureSection load='1gct' size='340' side='right'caption='[[1gct]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gct]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GCT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gct OCA], [https://pdbe.org/1gct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gct RCSB], [https://www.ebi.ac.uk/pdbsum/1gct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gct ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CTRA_BOVIN CTRA_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/1gct_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gct ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GCT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GCT OCA].
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*[[Chymotrypsin 3D structures|Chymotrypsin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Is gamma-chymotrypsin a tetrapeptide acyl-enzyme adduct of alpha-chymotrypsin?, Dixon MM, Matthews BW, Biochemistry. 1989 Aug 22;28(17):7033-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2819046 2819046]
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[[Category: Bos taurus]]
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[[Category: Chymotrypsin]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Dixon MM]]
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[[Category: Dixon, M.M.]]
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[[Category: Matthews BW]]
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[[Category: Matthews, B.W.]]
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[[Category: SO4]]
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[[Category: hydrolase (serine proteinase)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 15:57:03 2007''
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Current revision

IS GAMMA-CHYMOTRYPSIN A TETRAPEPTIDE ACYL-ENZYME ADDUCT OF GAMMA-CHYMOTRYPSIN?

PDB ID 1gct

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