1is9

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{{Seed}}
 
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[[Image:1is9.png|left|200px]]
 
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==Endoglucanase A from Clostridium thermocellum at atomic resolution==
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The line below this paragraph, containing "STRUCTURE_1is9", creates the "Structure Box" on the page.
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<StructureSection load='1is9' size='340' side='right'caption='[[1is9]], [[Resolution|resolution]] 1.03&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1is9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IS9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.03&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
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{{STRUCTURE_1is9| PDB=1is9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1is9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1is9 OCA], [https://pdbe.org/1is9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1is9 RCSB], [https://www.ebi.ac.uk/pdbsum/1is9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1is9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GUNA_ACET2 GUNA_ACET2] This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/is/1is9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1is9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the endoglucanase A from Clostridium thermocellum CelA was re-solved by three-wavelength MAD. Experimental phases were obtained in the resolution range 25-1.0 A. Various structure-solution approaches were tested in order to quantify the contribution of each wavelength. Two-wavelength MAD phasing was sufficient to obtain excellent experimental phases. SAD at the remote wavelength also resulted in interpretable maps. The three-wavelength MAD electron-density map was of excellent quality: for parts of the structure, atom types and bond types could be easily assigned. Double bonds in peptide links and side chains could be located owing to their increased electron density indicating their pi character. Comparison with a previously determined structure of CelA at 1.65 A showed that, apart from a few additional multiple conformers and differently oriented side chains, major differences occur at the protein-solvent interface. A complete additional solvent shell could be observed and the inner shells have been completed. The high accuracy of the structure allowed unambiguous assignment of the protonation state for the active-site catalytic carboxylates.
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===Endoglucanase A from Clostridium thermocellum at atomic resolution===
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Advantages of high-resolution phasing: MAD to atomic resolution.,Schmidt A, Gonzalez A, Morris RJ, Costabel M, Alzari PM, Lamzin VS Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1433-41. Epub 2002, Aug 23. PMID:12198299<ref>PMID:12198299</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1is9" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12198299}}, adds the Publication Abstract to the page
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12198299 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12198299}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Acetivibrio thermocellus]]
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1IS9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IS9 OCA].
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[[Category: Large Structures]]
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[[Category: Alzari PM]]
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==Reference==
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[[Category: Costabel M]]
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Advantages of high-resolution phasing: MAD to atomic resolution., Schmidt A, Gonzalez A, Morris RJ, Costabel M, Alzari PM, Lamzin VS, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1433-41. Epub 2002, Aug 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198299 12198299]
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[[Category: Gonzalez A]]
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[[Category: Cellulase]]
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[[Category: Lamzin VS]]
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[[Category: Clostridium thermocellum]]
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[[Category: Morris RJ]]
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[[Category: Single protein]]
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[[Category: Schmidt A]]
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[[Category: Alzari, P M.]]
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[[Category: Costabel, M.]]
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[[Category: Gonzalez, A.]]
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[[Category: Lamzin, V S.]]
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[[Category: Morris, R J.]]
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[[Category: Schmidt, A.]]
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[[Category: Endoglucanase family 8]]
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[[Category: Hg derivative]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:54:55 2008''
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Current revision

Endoglucanase A from Clostridium thermocellum at atomic resolution

PDB ID 1is9

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