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1gpc

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(New page: 200px<br /><applet load="1gpc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gpc, resolution 2.200&Aring;" /> '''CORE GP32, DNA-BIND...)
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[[Image:1gpc.gif|left|200px]]<br /><applet load="1gpc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gpc, resolution 2.200&Aring;" />
 
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'''CORE GP32, DNA-BINDING PROTEIN'''<br />
 
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==Overview==
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==CORE GP32, DNA-BINDING PROTEIN==
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The single-stranded DNA (ssDNA) binding protein gp32 from bacteriophage T4, is essential for T4 DNA replication, recombination and repair. In vivo, gp32 binds ssDNA as the replication fork advances and stimulates replisome, processivity and accuracy by a factor of several hundred. Gp32 binding, affects nearly every major aspect of DNA metabolism. Among its important, functions are: (1) configuring ssDNA templates for efficient use by the, replisome including DNA polymerase; (2) melting out adventitious secondary, structures; (3) protecting exposed ssDNA from nucleases; and (4), facilitating homologous recombination by binding ssDNA during strand, displacement. We have determined the crystal structure of the gp32 DNA, binding domain complexed to ssDNA at 2.2 A resolution. The ssDNA binding, cleft comprises regions from three structural subdomains and includes a, positively charged surface that runs parallel to a series of hydrophobic, pockets formed by clusters of aromatic side chains. Although only weak, electron density is seen for the ssDNA, it indicates that the phosphate, backbone contacts an electropositive cleft of the protein, placing the, bases in contact with the hydrophobic pockets. The DNA mobility implied by, the weak electron density may reflect the role of gp32 as a, sequence-independent ssDNA chaperone allowing the largely unstructured, ssDNA to slide freely through the cleft.
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<StructureSection load='1gpc' size='340' side='right'caption='[[1gpc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gpc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GPC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gpc OCA], [https://pdbe.org/1gpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gpc RCSB], [https://www.ebi.ac.uk/pdbsum/1gpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gpc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SSB_BPT4 SSB_BPT4] Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair (Probable). Coats the lagging-strand ssDNA as the replication fork advances (PubMed:9079662). Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor (PubMed:14871889). Together with DnaB-like SF4 replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange (PubMed:11459967). Promotes also the formation of joint molecules (PubMed:9079662). mRNA specific autogenous translational repressor (PubMed:15507125).[HAMAP-Rule:MF_04152]<ref>PMID:11459967</ref> <ref>PMID:14871889</ref> <ref>PMID:15507125</ref> <ref>PMID:9079662</ref> <ref>PMID:9079662</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gp/1gpc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gpc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1GPC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GPC OCA].
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) complexed to DNA., Shamoo Y, Friedman AM, Parsons MR, Konigsberg WH, Steitz TA, Nature. 1995 Jul 27;376(6538):362-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7630406 7630406]
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__TOC__
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[[Category: Bacteriophage t4]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Escherichia virus T4]]
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[[Category: Friedman, A.M.]]
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[[Category: Large Structures]]
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[[Category: Konigsberg, W.H.]]
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[[Category: Friedman AM]]
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[[Category: Parsons, M.R.]]
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[[Category: Konigsberg WH]]
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[[Category: Shamoo, Y.]]
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[[Category: Parsons MR]]
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[[Category: Steitz, T.A.]]
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[[Category: Shamoo Y]]
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[[Category: ZN]]
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[[Category: Steitz TA]]
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[[Category: single-stranded dna-binding]]
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[[Category: ssb]]
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[[Category: zinc binding domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:13:10 2007''
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Current revision

CORE GP32, DNA-BINDING PROTEIN

PDB ID 1gpc

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