1gr0

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(New page: 200px<br /><applet load="1gr0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gr0, resolution 1.95&Aring;" /> '''MYO-INOSITOL 1-PHOSP...)
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[[Image:1gr0.gif|left|200px]]<br /><applet load="1gr0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gr0, resolution 1.95&Aring;" />
 
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'''MYO-INOSITOL 1-PHOSPHATE SYNTHASE FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH NAD AND ZINC.'''<br />
 
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==Overview==
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==myo-inositol 1-phosphate synthase from Mycobacterium tuberculosis in complex with NAD and zinc.==
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Phosphatidylinositol (PI) is essential for Mycobacterium tuberculosis, viability and the enzymes involved in the PI biosynthetic pathway are, potential antimycobacterial agents for which little structural information, is available. The rate-limiting step in the pathway is the production of, (L)-myo-inositol 1-phosphate from (D)-glucose 6-phosphate, a complex, reaction catalyzed by the enzyme inositol 1-phosphate synthase. We have, determined the crystal structure of this enzyme from Mycobacterium, tuberculosis (tbINO) at 1.95 A resolution, bound to the cofactor NAD+. The, active site is located within a deep cleft at the junction between two, domains. The unexpected presence of a zinc ion here suggests a mechanistic, difference from the eukaryotic inositol synthases, which are stimulated by, monovalent cations, that may be exploitable in developing selective, inhibitors of tbINO.
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<StructureSection load='1gr0' size='340' side='right'caption='[[1gr0]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gr0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GR0 FirstGlance]. <br>
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1GR0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with ZN, CAC and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inositol-3-phosphate_synthase Inositol-3-phosphate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.4 5.5.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GR0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gr0 OCA], [https://pdbe.org/1gr0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gr0 RCSB], [https://www.ebi.ac.uk/pdbsum/1gr0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gr0 ProSAT], [https://www.topsan.org/Proteins/TBSGC/1gr0 TOPSAN]</span></td></tr>
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Crystal structure of inositol 1-phosphate synthase from Mycobacterium tuberculosis, a key enzyme in phosphatidylinositol synthesis., Norman RA, McAlister MS, Murray-Rust J, Movahedzadeh F, Stoker NG, McDonald NQ, Structure. 2002 Mar;10(3):393-402. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12005437 12005437]
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</table>
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[[Category: Inositol-3-phosphate synthase]]
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== Function ==
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[https://www.uniprot.org/uniprot/INO1_MYCTU INO1_MYCTU] Catalyzes the conversion of glucose 6-phosphate to 1D-myo-inositol 3-phosphate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gr/1gr0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gr0 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: McDonald NQ]]
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[[Category: Mcdonald, N.Q.]]
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[[Category: Murray-Rust J]]
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[[Category: Murray-Rust, J.]]
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[[Category: Norman RA]]
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[[Category: Norman, R.A.]]
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[[Category: TBSGC, TB.Structural.Genomics.Consortium.]]
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[[Category: CAC]]
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[[Category: NAD]]
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[[Category: ZN]]
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[[Category: oxidoreductase]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: synthase]]
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[[Category: tb]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:14:36 2007''
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Current revision

myo-inositol 1-phosphate synthase from Mycobacterium tuberculosis in complex with NAD and zinc.

PDB ID 1gr0

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