1gtq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1gtq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gtq, resolution 2.3&Aring;" /> '''6-PYRUVOYL TETRAHYDRO...)
Current revision (07:26, 7 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1gtq.gif|left|200px]]<br /><applet load="1gtq" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1gtq, resolution 2.3&Aring;" />
 
-
'''6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE'''<br />
 
-
==Overview==
+
==6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE==
-
The crystal structure of rat liver 6-pyruvoyl tetrahydropterin synthase, has been solved by multiple isomorphous replacement and refined to a, crystallographic R-factor of 20.4% at 2.3 A resolution. 6-Pyruvoyl, tetrahydrobiopterin synthase catalyses the conversion of dihydroneopterin, triphosphate to 6-pyruvoyl tetrahydropterin, the second of three enzymatic, steps in the synthesis of tetrahydrobiopterin from GTP. The functional, enzyme is a hexamer of identical subunits. The 6-pyruvoyl tetrahydropterin, synthase monomer folds into a sequential, four-stranded, antiparallel, beta-sheet with a 25 residue, helix-containing insertion between strands 1, and 2 at the bottom of the molecule, and a segment between strands 2 and 3, forming a pair of antiparallel helices, layered on one side of the, beta-sheet. Three 6-pyruvoyl tetrahydropterin synthase monomers form an, unusual 12-stranded antiparallel beta-barrel by tight association between, the N- and C-terminal beta-strands of two adjacent subunits. The barrel, encloses a highly basic pore of 6-12 A diameter. Two trimers associate in, a head-to-head fashion to form the active enzyme complex. The, substrate-binding site is located close to the trimer-trimer interface and, comprises residues from three monomers: A, A' and B. A metal-binding site, in the substrate-binding pocket is formed by the three histidine residues, 23, 48 and 50 from one 6-pyruvoyl tetrahydropterin synthase subunit. Close, to the metal, but apparently not liganding it, are residues Cys42, Glu133, (both from A) and His89 (from B), which might serve as proton donors and, acceptors during catalysis.
+
<StructureSection load='1gtq' size='340' side='right'caption='[[1gtq]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1gtq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus]. The August 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Tetrahydrobiopterin Biosynthesis'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_8 10.2210/rcsb_pdb/mom_2015_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GTQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GTQ FirstGlance]. <br>
-
1GTQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/6-pyruvoyltetrahydropterin_synthase 6-pyruvoyltetrahydropterin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.12 4.2.3.12] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GTQ OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gtq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gtq OCA], [https://pdbe.org/1gtq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gtq RCSB], [https://www.ebi.ac.uk/pdbsum/1gtq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gtq ProSAT]</span></td></tr>
-
Three-dimensional structure of 6-pyruvoyl tetrahydropterin synthase, an enzyme involved in tetrahydrobiopterin biosynthesis., Nar H, Huber R, Heizmann CW, Thony B, Burgisser D, EMBO J. 1994 Mar 15;13(6):1255-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8137809 8137809]
+
</table>
-
[[Category: 6-pyruvoyltetrahydropterin synthase]]
+
== Disease ==
 +
[https://www.uniprot.org/uniprot/PTPS_RAT PTPS_RAT] Note=Deficiency leads to phenylketonuria.
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PTPS_RAT PTPS_RAT] Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/1gtq_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gtq ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus rattus]]
[[Category: Rattus rattus]]
-
[[Category: Single protein]]
+
[[Category: Tetrahydrobiopterin Biosynthesis]]
-
[[Category: Buergisser, D.]]
+
[[Category: Buergisser D]]
-
[[Category: Heizmann, C.W.]]
+
[[Category: Heizmann CW]]
-
[[Category: Huber, R.]]
+
[[Category: Huber R]]
-
[[Category: Nar, H.]]
+
[[Category: Nar H]]
-
[[Category: Thoeny, B.]]
+
[[Category: Thoeny B]]
-
[[Category: ZN]]
+
-
[[Category: phosphate elimination]]
+
-
[[Category: pterine synthesis]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:17:12 2007''
+

Current revision

6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE

PDB ID 1gtq

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gtq"
Personal tools