1ja9

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{{Seed}}
 
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[[Image:1ja9.png|left|200px]]
 
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==Crystal structure of 1,3,6,8-tetrahydroxynaphthalene reductase in complex with NADPH and pyroquilon==
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The line below this paragraph, containing "STRUCTURE_1ja9", creates the "Structure Box" on the page.
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<StructureSection load='1ja9' size='340' side='right'caption='[[1ja9]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ja9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyricularia_grisea Pyricularia grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JA9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PYQ:PYROQUILON'>PYQ</scene></td></tr>
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{{STRUCTURE_1ja9| PDB=1ja9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ja9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ja9 OCA], [https://pdbe.org/1ja9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ja9 RCSB], [https://www.ebi.ac.uk/pdbsum/1ja9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ja9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9HFV6_MAGGR Q9HFV6_MAGGR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ja/1ja9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ja9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two short chain dehydrogenase/reductases mediate naphthol reduction reactions in fungal melanin biosynthesis. An X-ray structure of 1,3,6,8-tetrahydroxynaphthalene reductase (4HNR) complexed with NADPH and pyroquilon was determined for examining substrate and inhibitor specificities that differ from those of 1,3,8-trihydroxynaphthalene reductase (3HNR). The 1.5 A resolution structure allows for comparisons with the 1.7 A resolution structure of 3HNR complexed with the same ligands. The sequences of the two proteins are 46% identical, and they have the same fold. The 30-fold lower affinity of the 4HNR-NADPH complex for pyroquilon (a commercial fungicide that targets 3HNR) in comparison to that of the 3HNR-NADPH complex can be explained by unfavorable interactions between the anionic carboxyl group of the C-terminal Ile282 of 4HNR and CH and CH(2) groups of the inhibitor that are countered by favorable inhibitor interactions with 3HNR. 1,3,8-Trihydroxynaphthalene (3HN) and 1,3,6,8-tetrahydroxynaphthalene (4HN) were modeled onto the cyclic structure of pyroquilon in the 4HNR-NADPH-pyroquilon complex to examine the 300-fold preference of the enzyme for 4HN over 3HN. The models suggest that the C-terminal carboxyl group of Ile282 has a favorable hydrogen bonding interaction with the C6 hydroxyl group of 4HN and an unfavorable interaction with the C6 CH group of 3HN. Models of 3HN and 4HN in the 3HNR active site suggest a favorable interaction of the sulfur atom of the C-terminal Met283 with the C6 CH group of 3HN and an unfavorable one with the C6 hydroxyl group of 4HN, accounting for the 4-fold difference in substrate specificities. Thus, the C-terminal residues of the two naphthol reductase are determinants of inhibitor and substrate specificities.
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===Crystal structure of 1,3,6,8-tetrahydroxynaphthalene reductase in complex with NADPH and pyroquilon===
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A structural account of substrate and inhibitor specificity differences between two naphthol reductases.,Liao DI, Thompson JE, Fahnestock S, Valent B, Jordan DB Biochemistry. 2001 Jul 31;40(30):8696-704. PMID:11467929<ref>PMID:11467929</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_11467929}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1ja9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11467929 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11467929}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1JA9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JA9 OCA].
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[[Category: Pyricularia grisea]]
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[[Category: Fahnestock S]]
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==Reference==
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[[Category: Jordan DB]]
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A structural account of substrate and inhibitor specificity differences between two naphthol reductases., Liao DI, Thompson JE, Fahnestock S, Valent B, Jordan DB, Biochemistry. 2001 Jul 31;40(30):8696-704. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11467929 11467929]
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[[Category: Liao D-I]]
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[[Category: Magnaporthe grisea]]
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[[Category: Thompson JE]]
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[[Category: Single protein]]
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[[Category: Valent B]]
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[[Category: Fahnestock, S.]]
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[[Category: Jordan, D B.]]
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[[Category: Liao, D I.]]
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[[Category: Thompson, J E.]]
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[[Category: Valent, B.]]
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[[Category: Oxidoreductase]]
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[[Category: Protein-nadph-active site inhibitor complex]]
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[[Category: Short chain dehydrogenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:58:25 2008''
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Current revision

Crystal structure of 1,3,6,8-tetrahydroxynaphthalene reductase in complex with NADPH and pyroquilon

PDB ID 1ja9

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