1h8g

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(New page: 200px<br /><applet load="1h8g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h8g, resolution 2.40&Aring;" /> '''C-TERMINAL DOMAIN OF...)
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[[Image:1h8g.jpg|left|200px]]<br /><applet load="1h8g" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h8g, resolution 2.40&Aring;" />
 
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'''C-TERMINAL DOMAIN OF THE MAJOR AUTOLYSIN (C-LYTA) FROM STREPTOCOCCUS PNEUMONIAE'''<br />
 
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==Overview==
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==C-terminal domain of the major autolysin (C-LytA) from Streptococcus pneumoniae==
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Choline binding proteins are virulence determinants present in several, Gram-positive bacteria. Because anchorage of these proteins to the cell, wall through their choline binding domain is essential for bacterial, virulence, their release from the cell surface is considered a powerful, target for a weapon against these pathogens. The first crystal structure, of a choline binding domain, from the toxin-releasing enzyme pneumococcal, major autolysin (LytA), reveals a novel solenoid fold consisting, exclusively of beta-hairpins that stack to form a left-handed superhelix., This unique structure is maintained by choline molecules at the, hydrophobic interface of consecutive hairpins and may be present in other, choline binding proteins that share high homology to the repeated motif of, the domain.
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<StructureSection load='1h8g' size='340' side='right'caption='[[1h8g]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1h8g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H8G FirstGlance]. <br>
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1H8G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with CHT as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N-acetylmuramoyl-L-alanine_amidase N-acetylmuramoyl-L-alanine amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.28 3.5.1.28] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8G OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h8g OCA], [https://pdbe.org/1h8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h8g RCSB], [https://www.ebi.ac.uk/pdbsum/1h8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h8g ProSAT]</span></td></tr>
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A novel solenoid fold in the cell wall anchoring domain of the pneumococcal virulence factor LytA., Fernandez-Tornero C, Lopez R, Garcia E, Gimenez-Gallego G, Romero A, Nat Struct Biol. 2001 Dec;8(12):1020-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11694890 11694890]
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</table>
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[[Category: N-acetylmuramoyl-L-alanine amidase]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/ALYS_STRPN ALYS_STRPN] Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/1h8g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h8g ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
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[[Category: Fernandez-Tornero, C.]]
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[[Category: Fernandez-Tornero C]]
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[[Category: Garcia, E.]]
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[[Category: Garcia E]]
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[[Category: Gimenez-Gallego, G.]]
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[[Category: Gimenez-Gallego G]]
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[[Category: Lopez, R.]]
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[[Category: Lopez R]]
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[[Category: Romero, A.]]
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[[Category: Romero A]]
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[[Category: CHT]]
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[[Category: cell wall attachment]]
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[[Category: choline-binding domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:27:37 2007''
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Current revision

C-terminal domain of the major autolysin (C-LytA) from Streptococcus pneumoniae

PDB ID 1h8g

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