1ha6

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(New page: 200px<br /><applet load="1ha6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ha6" /> '''NMR SOLUTION STRUCTURE OF MURINE CCL20/MIP-3...)
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[[Image:1ha6.jpg|left|200px]]<br /><applet load="1ha6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ha6" />
 
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'''NMR SOLUTION STRUCTURE OF MURINE CCL20/MIP-3A CHEMOKINE'''<br />
 
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==Overview==
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==NMR Solution Structure of Murine CCL20/MIP-3a Chemokine==
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CCL20/MIP-3alpha is a beta-chemokine expressed in the thymus, skin, and, intestinal epithelial cells that exclusively binds and activates the CCR6, receptor in both mice and humans. The strict receptor binding specificity, of CCL20 is exceptional; other chemokines and their receptors bind, promiscuously with multiple partners. Toward determining the structural, basis for the selective receptor specificity of CCL20, we have determined, its three-dimensional structure by 1H NMR spectroscopy. CCL20 exhibits the, same monomeric structure previously described for other chemokines: a, three-stranded beta-sheet and an overlying alpha-helix. The CCL20 receptor, selectivity could arise from the rigid conformation of the N-terminal DCCL, motif as well as the groove between the N-loop and the beta2-beta3, hairpin, which is significantly narrower in CCL20 than in other, chemokines. Similar structural features are seen in human beta-defensin 2, a small nonchemokine polypeptide reported to selectively bind and activate, CCR6, which stresses their importance for the specific binding of both, CCL20 and beta-defensin 2 to CCR6. CCL20's structure will be useful to, design tools aimed to modulate its important biological functions.
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<StructureSection load='1ha6' size='340' side='right'caption='[[1ha6]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ha6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HA6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HA6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ha6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ha6 OCA], [https://pdbe.org/1ha6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ha6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ha6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ha6 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ha/1ha6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ha6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CCL20/MIP-3alpha is a beta-chemokine expressed in the thymus, skin, and intestinal epithelial cells that exclusively binds and activates the CCR6 receptor in both mice and humans. The strict receptor binding specificity of CCL20 is exceptional; other chemokines and their receptors bind promiscuously with multiple partners. Toward determining the structural basis for the selective receptor specificity of CCL20, we have determined its three-dimensional structure by 1H NMR spectroscopy. CCL20 exhibits the same monomeric structure previously described for other chemokines: a three-stranded beta-sheet and an overlying alpha-helix. The CCL20 receptor selectivity could arise from the rigid conformation of the N-terminal DCCL motif as well as the groove between the N-loop and the beta2-beta3 hairpin, which is significantly narrower in CCL20 than in other chemokines. Similar structural features are seen in human beta-defensin 2, a small nonchemokine polypeptide reported to selectively bind and activate CCR6, which stresses their importance for the specific binding of both CCL20 and beta-defensin 2 to CCR6. CCL20's structure will be useful to design tools aimed to modulate its important biological functions.
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==About this Structure==
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NMR solution structure of murine CCL20/MIP-3alpha, a chemokine that specifically chemoattracts immature dendritic cells and lymphocytes through its highly specific interaction with the beta-chemokine receptor CCR6.,Perez-Canadillas JM, Zaballos A, Gutierrez J, Varona R, Roncal F, Albar JP, Marquez G, Bruix M J Biol Chem. 2001 Jul 27;276(30):28372-9. Epub 2001 May 23. PMID:11373289<ref>PMID:11373289</ref>
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1HA6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HA6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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NMR solution structure of murine CCL20/MIP-3alpha, a chemokine that specifically chemoattracts immature dendritic cells and lymphocytes through its highly specific interaction with the beta-chemokine receptor CCR6., Perez-Canadillas JM, Zaballos A, Gutierrez J, Varona R, Roncal F, Albar JP, Marquez G, Bruix M, J Biol Chem. 2001 Jul 27;276(30):28372-9. Epub 2001 May 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11373289 11373289]
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</div>
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<div class="pdbe-citations 1ha6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Albar JP]]
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[[Category: Albar, J.P.]]
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[[Category: Bruix M]]
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[[Category: Bruix, M.]]
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[[Category: Gutierrez J]]
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[[Category: Gutierrez, J.]]
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[[Category: Marquez G]]
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[[Category: Marquez, G.]]
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[[Category: Perez-Canadillas JM]]
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[[Category: Perez-Canadillas, J.M.]]
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[[Category: Roncal F]]
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[[Category: Roncal, F.]]
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[[Category: Varona R]]
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[[Category: Varona, R.]]
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[[Category: Zaballos A]]
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[[Category: Zaballos, A.]]
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[[Category: ccl20/mip-3a]]
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[[Category: chemiokine]]
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[[Category: defensins]]
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[[Category: mouse]]
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[[Category: nmr]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:28:44 2007''
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Current revision

NMR Solution Structure of Murine CCL20/MIP-3a Chemokine

PDB ID 1ha6

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