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| - | {{Seed}} | |
| - | [[Image:1jnb.png|left|200px]] | |
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| - | <!--
| + | ==CONNECTOR PROTEIN FROM BACTERIOPHAGE PHI29== |
| - | The line below this paragraph, containing "STRUCTURE_1jnb", creates the "Structure Box" on the page.
| + | <StructureSection load='1jnb' size='340' side='right'caption='[[1jnb]], [[Resolution|resolution]] 3.20Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1jnb]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JNB FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jnb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jnb OCA], [https://pdbe.org/1jnb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jnb RCSB], [https://www.ebi.ac.uk/pdbsum/1jnb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jnb ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1jnb| PDB=1jnb | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | ===CONNECTOR PROTEIN FROM BACTERIOPHAGE PHI29===
| + | [https://www.uniprot.org/uniprot/PORTL_BPPH2 PORTL_BPPH2] Forms the portal vertex of the capsid (PubMed:10801350) (PubMed:19744688, PubMed:21570409). This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection (By similarity). The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel (PubMed:11812138, PubMed:21570409). Binds to the 6 packaging RNA molecules (pRNA) forming a double-ring structure which in turn binds to the ATPase gp16 hexamer, forming the active DNA-translocating motor (PubMed:15886394, PubMed:11130079). This complex is essential for the specificity of packaging from the left DNA end.[UniProtKB:P13334]<ref>PMID:11130079</ref> <ref>PMID:11812138</ref> <ref>PMID:15886394</ref> <ref>PMID:19744688</ref> <ref>PMID:21570409</ref> <ref>PMID:10801350</ref> |
| - | | + | == References == |
| - | | + | <references/> |
| - | <!-- | + | __TOC__ |
| - | The line below this paragraph, {{ABSTRACT_PUBMED_11526317}}, adds the Publication Abstract to the page
| + | </StructureSection> |
| - | (as it appears on PubMed at http://www.pubmed.gov), where 11526317 is the PubMed ID number.
| + | [[Category: Bacillus virus phi29]] |
| - | -->
| + | [[Category: Large Structures]] |
| - | {{ABSTRACT_PUBMED_11526317}}
| + | [[Category: Anderson DL]] |
| - | | + | [[Category: Badasso MO]] |
| - | ==About this Structure== | + | [[Category: He Y]] |
| - | 1JNB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_phage_phi29 Bacillus phage phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNB OCA].
| + | [[Category: Jardine PJ]] |
| - | | + | [[Category: Leiman PG]] |
| - | ==Reference== | + | [[Category: Rossmann MG]] |
| - | Structure determination of the head-tail connector of bacteriophage phi29., Simpson AA, Leiman PG, Tao Y, He Y, Badasso MO, Jardine PJ, Anderson DL, Rossmann MG, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1260-9. Epub 2001, Aug 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11526317 11526317]
| + | [[Category: Simpson AA]] |
| - | [[Category: Bacillus phage phi29]] | + | [[Category: Tao Y]] |
| - | [[Category: Single protein]] | + | |
| - | [[Category: Anderson, D L.]] | + | |
| - | [[Category: Badasso, M O.]] | + | |
| - | [[Category: He, Y.]] | + | |
| - | [[Category: Jardine, P J.]] | + | |
| - | [[Category: Leiman, P G.]] | + | |
| - | [[Category: Rossmann, M G.]] | + | |
| - | [[Category: Simpson, A A.]] | + | |
| - | [[Category: Tao, Y.]] | + | |
| - | [[Category: Helix bundle]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:30:06 2008''
| + | |
| Structural highlights
Function
PORTL_BPPH2 Forms the portal vertex of the capsid (PubMed:10801350) (PubMed:19744688, PubMed:21570409). This portal plays critical roles in head assembly, genome packaging, neck/tail attachment, and genome ejection (By similarity). The portal protein multimerizes as a single ring-shaped homododecamer arranged around a central channel (PubMed:11812138, PubMed:21570409). Binds to the 6 packaging RNA molecules (pRNA) forming a double-ring structure which in turn binds to the ATPase gp16 hexamer, forming the active DNA-translocating motor (PubMed:15886394, PubMed:11130079). This complex is essential for the specificity of packaging from the left DNA end.[UniProtKB:P13334][1] [2] [3] [4] [5] [6]
References
- ↑ Simpson AA, Tao Y, Leiman PG, Badasso MO, He Y, Jardine PJ, Olson NH, Morais MC, Grimes S, Anderson DL, Baker TS, Rossmann MG. Structure of the bacteriophage phi29 DNA packaging motor. Nature. 2000 Dec 7;408(6813):745-50. PMID:11130079 doi:10.1038/35047129
- ↑ Guasch A, Pous J, Ibarra B, Gomis-Ruth FX, Valpuesta JM, Sousa N, Carrascosa JL, Coll M. Detailed architecture of a DNA translocating machine: the high-resolution structure of the bacteriophage phi29 connector particle. J Mol Biol. 2002 Jan 25;315(4):663-76. PMID:11812138 doi:http://dx.doi.org/10.1006/jmbi.2001.5278
- ↑ Xiao F, Moll WD, Guo S, Guo P. Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29. Nucleic Acids Res. 2005 May 10;33(8):2640-9. doi: 10.1093/nar/gki554. Print 2005. PMID:15886394 doi:http://dx.doi.org/10.1093/nar/gki554
- ↑ Fu CY, Prevelige PE Jr. In vitro incorporation of the phage Phi29 connector complex. Virology. 2009 Nov 10;394(1):149-53. doi: 10.1016/j.virol.2009.08.016. Epub 2009 , Sep 9. PMID:19744688 doi:http://dx.doi.org/10.1016/j.virol.2009.08.016
- ↑ Grimes S, Ma S, Gao J, Atz R, Jardine PJ. Role of phi29 connector channel loops in late-stage DNA packaging. J Mol Biol. 2011 Jul 1;410(1):50-9. doi: 10.1016/j.jmb.2011.04.070. Epub 2011 May, 5. PMID:21570409 doi:http://dx.doi.org/10.1016/j.jmb.2011.04.070
- ↑ Ibarra B, Caston JR, Llorca O, Valle M, Valpuesta JM, Carrascosa JL. Topology of the components of the DNA packaging machinery in the phage phi29 prohead. J Mol Biol. 2000 May 19;298(5):807-15. doi: 10.1006/jmbi.2000.3712. PMID:10801350 doi:http://dx.doi.org/10.1006/jmbi.2000.3712
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