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1hg8

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(New page: 200px<br /><applet load="1hg8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hg8, resolution 1.73&Aring;" /> '''ENDOPOLYGALACTURONAS...)
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[[Image:1hg8.gif|left|200px]]<br /><applet load="1hg8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hg8, resolution 1.73&Aring;" />
 
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'''ENDOPOLYGALACTURONASE FROM THE PHYTOPATHOGENIC FUNGUS FUSARIUM MONILIFORME'''<br />
 
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==Overview==
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==Endopolygalacturonase from the phytopathogenic fungus Fusarium moniliforme==
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To invade a plant tissue, phytopathogenic fungi produce several cell, wall-degrading enzymes; among them, endopolygalacturonase (PG) catalyzes, the fragmentation and solubilization of homogalacturonan., Polygalacturonase-inhibiting proteins (PGIPs), found in the cell wall of, many plants, counteract fungal PGs by forming specific complexes with, them. We report the crystal structure at 1.73 A resolution of PG from the, phytopathogenic fungus Fusarium moniliforme (FmPG). The structure of FmPG, was useful to study the mode of interaction of the enzyme with PGIP-2 from, Phaseolus vulgaris. Several amino acids of FmPG were mutated, and their, contribution to the formation of the complex with PGIP-2 was investigated, by surface plasmon resonance. The residues Lys-269 and Arg-267, located, inside the active site cleft, and His-188, at the edge of the active site, cleft, are critical for the formation of the complex, which is consistent, with the observed competitive inhibition of the enzyme played by PGIP-2., The replacement of His-188 with a proline or the insertion of a tryptophan, after position 270, variations that both occur in plant PGs, interferes, with the formation of the complex. We suggest that these variations are, important structural requirements of plant PGs to prevent PGIP binding.
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<StructureSection load='1hg8' size='340' side='right'caption='[[1hg8]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1hg8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_verticillioides Fusarium verticillioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HG8 FirstGlance]. <br>
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1HG8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gibberella_moniliformis Gibberella moniliformis] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HG8 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hg8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hg8 OCA], [https://pdbe.org/1hg8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hg8 RCSB], [https://www.ebi.ac.uk/pdbsum/1hg8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hg8 ProSAT]</span></td></tr>
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Structural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein)., Federici L, Caprari C, Mattei B, Savino C, Di Matteo A, De Lorenzo G, Cervone F, Tsernoglou D, Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13425-30. Epub 2001 Oct 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11687632 11687632]
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</table>
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[[Category: Gibberella moniliformis]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/PGLR_GIBFU PGLR_GIBFU] Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.
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[[Category: Caprari, C.]]
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== Evolutionary Conservation ==
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[[Category: Cervone, F.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Federici, L.]]
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Check<jmol>
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[[Category: Lorenzo, G.De.]]
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<jmolCheckbox>
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[[Category: Mattei, B.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hg/1hg8_consurf.spt"</scriptWhenChecked>
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[[Category: Savino, C.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Tsernoglou, D.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: NAG]]
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</jmolCheckbox>
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[[Category: hydrolase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hg8 ConSurf].
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[[Category: pectin degradation]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:34:45 2007''
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</StructureSection>
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[[Category: Fusarium verticillioides]]
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[[Category: Large Structures]]
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[[Category: Caprari C]]
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[[Category: Cervone F]]
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[[Category: De Lorenzo G]]
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[[Category: Federici L]]
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[[Category: Mattei B]]
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[[Category: Savino C]]
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[[Category: Tsernoglou D]]

Current revision

Endopolygalacturonase from the phytopathogenic fungus Fusarium moniliforme

PDB ID 1hg8

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