1hno

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(New page: 200px<br /><applet load="1hno" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hno, resolution 2.5&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1hno.jpg|left|200px]]<br /><applet load="1hno" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hno, resolution 2.5&Aring;" />
 
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'''CRYSTAL STRUCTURE OF PEROXISOMAL DELTA3-DELTA2-ENOYL-COA ISOMERASE FROM SACCHAROMYCES CEREVISIAE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF PEROXISOMAL DELTA3-DELTA2-ENOYL-COA ISOMERASE FROM SACCHAROMYCES CEREVISIAE==
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The active-site geometry of the first crystal structure of a, Delta(3)-Delta(2)-enoyl-coenzyme A (CoA) isomerase (the peroxisomal enzyme, from the yeast Saccharomyces cerevisiae) shows that only one catalytic, base, Glu158, is involved in shuttling the proton from the C2 carbon atom, of the substrate, Delta(3)-enoyl-CoA, to the C4 atom of the product, Delta(2)-enoyl-CoA. Site-directed mutagenesis has been performed to, confirm that this glutamate residue is essential for catalysis. This, Delta(3)-Delta(2)-enoyl-CoA isomerase is a hexameric enzyme, consisting of, six identical subunits. It belongs to the hydratase/isomerase superfamily, of enzymes which catalyze a wide range of CoA-dependent reactions. The, members of the hydratase/ isomerase superfamily have only a low level of, sequence identity. Comparison of the crystal structure of the, Delta(3)-Delta(2)-enoyl-CoA isomerase with the other structures of this, superfamily shows only one region of large structural variability, which, is in the second turn of the spiral fold and which is involved in defining, the shape of the binding pocket.
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<StructureSection load='1hno' size='340' side='right'caption='[[1hno]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1hno]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HNO FirstGlance]. <br>
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1HNO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with EDO as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Dodecenoyl-CoA_isomerase Dodecenoyl-CoA isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.8 5.3.3.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HNO OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hno OCA], [https://pdbe.org/1hno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hno RCSB], [https://www.ebi.ac.uk/pdbsum/1hno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hno ProSAT]</span></td></tr>
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The crystal structure of delta(3)-delta(2)-enoyl-CoA isomerase., Mursula AM, van Aalten DM, Hiltunen JK, Wierenga RK, J Mol Biol. 2001 Jun 15;309(4):845-53. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11399063 11399063]
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</table>
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[[Category: Dodecenoyl-CoA isomerase]]
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== Function ==
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[https://www.uniprot.org/uniprot/ECI1_YEAST ECI1_YEAST] Essential for the beta oxidation of unsaturated fatty acids.<ref>PMID:9837886</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hn/1hno_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hno ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Hiltunen JK]]
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[[Category: Aalten, D.M.F.van.]]
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[[Category: Mursula AM]]
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[[Category: Hiltunen, J.K.]]
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[[Category: Wierenga RK]]
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[[Category: Mursula, A.M.]]
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[[Category: Van Aalten DMF]]
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[[Category: Wierenga, R.K.]]
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[[Category: EDO]]
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[[Category: alpha/beta]]
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[[Category: unliganded]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:40:40 2007''
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Current revision

CRYSTAL STRUCTURE OF PEROXISOMAL DELTA3-DELTA2-ENOYL-COA ISOMERASE FROM SACCHAROMYCES CEREVISIAE

PDB ID 1hno

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