1k9y

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{{Seed}}
 
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[[Image:1k9y.png|left|200px]]
 
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==The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate==
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The line below this paragraph, containing "STRUCTURE_1k9y", creates the "Structure Box" on the page.
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<StructureSection load='1k9y' size='340' side='right'caption='[[1k9y]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1k9y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K9Y FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_1k9y| PDB=1k9y | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k9y OCA], [https://pdbe.org/1k9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k9y RCSB], [https://www.ebi.ac.uk/pdbsum/1k9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k9y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MET22_YEAST MET22_YEAST] Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Involved in salt tolerance. Confers resistance to lithium.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k9/1k9y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k9y ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Li(+)-sensitive/Mg(2+)-dependent phosphatases have attracted considerable attention since they have been proposed as targets for lithium therapy in the treatment of manic-depressive patients. The members of this enzyme superfamily display low levels of sequence identity while possessing a common fold and active site. Extensive structural and biochemical data demonstrate the direct involvement of two metal ions in catalysis, and show that lithium exerts its inhibitory action by blocking the products at the active site. By exploiting the different inhibitory properties of magnesium and calcium, we have been able to solve the X-ray structures of the Li(+)-sensitive/Mg(2+)-dependent 3'-phosphoadenosine-5'-phosphatase in complex with its substrate and with its products. The structural comparison of these complexes provides a 3D picture of the different stages of the catalytic cycle. This gives new insights into the understanding of the biological function of this group of enzymes and their lithium inhibition, and should assist in the design of improved inhibitors of therapeutic value.
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===The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate===
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Structural enzymology of Li(+)-sensitive/Mg(2+)-dependent phosphatases.,Patel S, Martinez-Ripoll M, Blundell TL, Albert A J Mol Biol. 2002 Jul 26;320(5):1087-94. PMID:12126627<ref>PMID:12126627</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12126627}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1k9y" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12126627 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12126627}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1K9Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K9Y OCA].
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==Reference==
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Structural enzymology of Li(+)-sensitive/Mg(2+)-dependent phosphatases., Patel S, Martinez-Ripoll M, Blundell TL, Albert A, J Mol Biol. 2002 Jul 26;320(5):1087-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12126627 12126627]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Albert A]]
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[[Category: Albert, A.]]
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[[Category: Blundell TL]]
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[[Category: Blundell, T L.]]
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[[Category: Patel S]]
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[[Category: Patel, S.]]
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[[Category: Inositol]]
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[[Category: Nucleotidase]]
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[[Category: Salt tolerance]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:00:52 2008''
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Current revision

The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate

PDB ID 1k9y

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