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1khp

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{{Seed}}
 
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[[Image:1khp.png|left|200px]]
 
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==Monoclinic form of papain/ZLFG-DAM covalent complex==
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The line below this paragraph, containing "STRUCTURE_1khp", creates the "Structure Box" on the page.
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<StructureSection load='1khp' size='340' side='right'caption='[[1khp]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1khp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KHP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0HQ:DIAZOMETHANE'>0HQ</scene>, <scene name='pdbligand=PHQ:BENZYL+CHLOROCARBONATE'>PHQ</scene></td></tr>
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{{STRUCTURE_1khp| PDB=1khp | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1khp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1khp OCA], [https://pdbe.org/1khp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1khp RCSB], [https://www.ebi.ac.uk/pdbsum/1khp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1khp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PAPA1_CARPA PAPA1_CARPA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kh/1khp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1khp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional structure of two polymorphs of a ZLFG-CH2-papain covalent complex has been determined by X-ray crystallography. The structures indicate that: (i) the methylene carbon atom of the inhibitor is covalently bound to the Sgamma atom of Cys25 of papain; (ii) the hydrophobic S2 pocket formed by Pro68, Val133, Val157, and Asp158 is occupied by the inhibitor's phenylalanyl P2 side chain; (iii) extensive hydrogen bonding and hydrophobic interactions are responsible for the interaction of the inhibitor with the enzyme. Comparison with similar structures suggests that in covalent complexes preservation of main chain-main chain interactions between the enzyme and the inhibitor may have higher priority than the P-S interactions.
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===Monoclinic form of papain/ZLFG-DAM covalent complex===
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Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor.,Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M J Pept Res. 2004 Oct;64(4):141-50. PMID:15357669<ref>PMID:15357669</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1khp" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15357669}}, adds the Publication Abstract to the page
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*[[Papain|Papain]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15357669 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15357669}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1KHP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Carica_papaya Carica papaya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHP OCA].
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==Reference==
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Two polymorphs of a covalent complex between papain and a diazomethylketone inhibitor., Janowski R, Kozak M, Jankowska E, Grzonka Z, Jaskolski M, J Pept Res. 2004 Oct;64(4):141-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15357669 15357669]
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[[Category: Carica papaya]]
[[Category: Carica papaya]]
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[[Category: Papain]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Grzonka Z]]
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[[Category: Grzonka, Z.]]
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[[Category: Jankowska E]]
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[[Category: Jankowska, E.]]
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[[Category: Janowski R]]
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[[Category: Janowski, R.]]
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[[Category: Jaskolski M]]
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[[Category: Jaskolski, M.]]
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[[Category: Kozak M]]
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[[Category: Kozak, M.]]
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[[Category: Diazomethylketone inhibitor]]
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[[Category: Irreversible inhibitor]]
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[[Category: Protease inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:21:48 2008''
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Current revision

Monoclinic form of papain/ZLFG-DAM covalent complex

PDB ID 1khp

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